, Volume 22, Issue 3, pp 218-223
Date: 05 Sep 2010

Detection of human papilloma virus type 16 E6 mRNA in laryngeal squamous cell carcinoma by in situ hybridization

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Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor in Northeast China and is frequently associated with well-established risk factors like smoking and alcohol abuse. Human papilloma virus (HPV) is an epitheliotropic oncogenic virus that has been detected in a variety of head and neck tumors including LSCC. This retrospective study was to investigate the prevalence of HPV infection in patients with LSCC.


In situ hybridization was performed in 99 patients with LSCC to detect the expression of HPV-16 E6 mRNA.


The positive rate of HPV16 E6 mRNA was 36.36% (36/99) in laryngeal squamous cell carcinoma (LSCC), whereas only 3 of 50 (6%) specimens of the normal laryngeal mucosa as a control group showed positive results (P<0.05).Additionally, there was no corelation between HPV16 and age, gender, clinical stage, nodal status and tumor site (P>0.05).


The results suggest that the increased prevalence of HPV infection compared to normal laryngeal mucosa and the fact that high-risk HPV types (especially type 16) were the most frequently identified do not allow the exclusion of HPV as a risk factor in laryngeal squamous cell carcinoma. However, their clinical value remains to be further investigated.