Original Article

Chinese Journal of Cancer Research

, Volume 22, Issue 2, pp 141-147

First online:

Triptolide inhibits cell growth and induces G0- G1 arrest by regulating P21wap1/cip1 and P27 kip1 in human multiple myeloma RPMI-8226 cells

  • Yuan LiuAffiliated withInstitute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
  • , Ling-lan ZengAffiliated withInstitute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
  • , Yan ChenAffiliated withInstitute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
  • , Fei ZhaoAffiliated withInstitute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
  • , Rui LiAffiliated withInstitute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
  • , Chun ZhangAffiliated withInstitute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology Email author 
  • , Lu WenAffiliated withInstitute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Objective

To investigate the effects of triptolide(TPL) on cell growth, cell cycle and the expressions of p21wap1/cip1 and p27kip1.

Methods

MTT assay was used to determine the cell viability after triptolide treatment in human multiple myeloma RPMI-8226 cells. The effect on cell cycle distribution was determined by flow cytometry. Semi-quantitative reverse transcription-PCR was used to examine the mRNA expressions of p21wap1/cip1 and p27kip1. The protein expressions of p21 wap1/cip1 and p27kip1 were determined by Western blot.

Results

Triptolide of varying concentrations induced cell viability inhibition in dose- and time-related fashion and caused G0-G1 phase arrest of cell cycle progression in RPMI-8226 cells. These effects accompanied with up-modulation of the expressions of p21 wap1/cip1 and p27kip1.

Conclusion

These results suggest that triptolide inhibit cell proliferation and cell cycle progression via up-regulating p21wap1/cip1 and p27kip1 and triptolide may exert its anti-cancer activity through this pathway.

Key words

Triptolide RPMI-8226 cells Cell cycle Multiple myeloma

CLC number

R733.3