Chinese Journal of Cancer Research

, Volume 19, Issue 1, pp 1–6

Design of dendrimer modified carbon nanotubes for gene delivery

  • Pan Bi-feng 潘碧峰
  • Cui Da-xiang 崔大祥
  • Xu Ping 徐萍
  • Chen Hao 陈浩
  • Liu Feng-tao 刘凤涛
  • Li Qing 李清
  • Huang Tuo 黄拓
  • You Xiao-gang 尤晓刚
  • Shao Jun 邵君
  • Bao Chen-chen 鲍晨晨
  • Gao Feng 高峰
  • He Rong 贺蓉
  • Shu Meng-jun 舒孟军
  • Ma Yong-jie 马勇杰
Article

DOI: 10.1007/s11670-007-0001-0

Cite this article as:
Pan, B., Cui, D., Xu, P. et al. Chin. J. Cancer Res. (2007) 19: 1. doi:10.1007/s11670-007-0001-0

Abstract

Objective

To investigate the efficiency of polyamidoamine dendrimer grafted carbon nanotube (dendrimer-CNT) mediated entrance of anti-survivin oligonucleotide into MCF-7 cells, and its effects on the growth of MCF-7 cells.

Methods

Antisense survivin oligonucleotide was anchored onto polyamidoamine dendrimer grafted carbon nanotubes to form dendrimer-CNT-asODN complex and the complex was characterized by Zeta potential, AFM, TEM, and 1% agarose gel electrophoresis analysis. Dendrimer-CNT-asODN complexes were added into the medium and incubated with MCF-7 cells. MTT method was used to detect the effects of asODN and dendrimer-CNT-asODN on the growth of MCF-7 cells. TEM was used to observe the distribution of dendrimer-CNT-asODN complex within MCF-7 cells.

Results

Successful synthesis of dendrimer-CNT-asODN complexes was proved by TEM, AFM and agarose gel electrophoresis. TEM showed that the complexes were located in the cytoplasm, endosome, and lysosome within MCF-7 cells. When dendrimer-CNT-asODN (1.0 μmol/L) and asODN (1.0 μmol/L) were used for 120 h incubation, the inhibitory rates of MCF-7 cells were (28.22±3.5)% for dendrimer-CNT-asODN complex group, (9.23±0.56)% for only asODN group, and (3.44±0.25)% for dendrimer-CNT group. Dendrimer-CNT-asODN complex at 3.0 μmol/L inhibited MCF-7 cells by (30.30±10.62)%, and the inhibitory effects were in a time-and concentration-dependent manner.

Conclusion

Dendrimer-CNT nanoparticles may serve as a gene delivery vector with high efficiency, which can bring foreign gene into cancer cells, inhibiting cancer cell proliferation and markedly enhancing the cancer therapy effects.

Key words

Gene deliveryCarbon nanotubePolyamidoamine dendrimerCancer therapySurvivin gene

CLC number

R73-36+2

Copyright information

© Chinese Anti-Cancer Association 2007

Authors and Affiliations

  • Pan Bi-feng 潘碧峰
    • 1
  • Cui Da-xiang 崔大祥
    • 1
  • Xu Ping 徐萍
    • 1
  • Chen Hao 陈浩
    • 1
  • Liu Feng-tao 刘凤涛
    • 1
  • Li Qing 李清
    • 1
  • Huang Tuo 黄拓
    • 1
  • You Xiao-gang 尤晓刚
    • 1
  • Shao Jun 邵君
    • 1
  • Bao Chen-chen 鲍晨晨
    • 1
  • Gao Feng 高峰
    • 1
  • He Rong 贺蓉
    • 1
  • Shu Meng-jun 舒孟军
    • 1
  • Ma Yong-jie 马勇杰
    • 1
  1. 1.Department of Bio-Nano Science and Engineering, Key Lab for Thin Film and Micro fabrication of Ministry of Education, State Key Lab of Micro-Nano Fabrication Technology, Institute of Micro-Nano Science and TechnologyShanghai Jiao Tong UniversityShanghaiChina