Article

Chinese Journal of Cancer Research

, Volume 19, Issue 1, pp 1-6

Design of dendrimer modified carbon nanotubes for gene delivery

  • Pan Bi-feng 潘碧峰Affiliated withDepartment of Bio-Nano Science and Engineering, Key Lab for Thin Film and Micro fabrication of Ministry of Education, State Key Lab of Micro-Nano Fabrication Technology, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University
  • , Cui Da-xiang 崔大祥Affiliated withDepartment of Bio-Nano Science and Engineering, Key Lab for Thin Film and Micro fabrication of Ministry of Education, State Key Lab of Micro-Nano Fabrication Technology, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University Email author 
  • , Xu Ping 徐萍Affiliated withDepartment of Bio-Nano Science and Engineering, Key Lab for Thin Film and Micro fabrication of Ministry of Education, State Key Lab of Micro-Nano Fabrication Technology, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University
  • , Chen Hao 陈浩Affiliated withDepartment of Bio-Nano Science and Engineering, Key Lab for Thin Film and Micro fabrication of Ministry of Education, State Key Lab of Micro-Nano Fabrication Technology, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University
  • , Liu Feng-tao 刘凤涛Affiliated withDepartment of Bio-Nano Science and Engineering, Key Lab for Thin Film and Micro fabrication of Ministry of Education, State Key Lab of Micro-Nano Fabrication Technology, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University
  • , Li Qing 李清Affiliated withDepartment of Bio-Nano Science and Engineering, Key Lab for Thin Film and Micro fabrication of Ministry of Education, State Key Lab of Micro-Nano Fabrication Technology, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University
  • , Huang Tuo 黄拓Affiliated withDepartment of Bio-Nano Science and Engineering, Key Lab for Thin Film and Micro fabrication of Ministry of Education, State Key Lab of Micro-Nano Fabrication Technology, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University
  • , You Xiao-gang 尤晓刚Affiliated withDepartment of Bio-Nano Science and Engineering, Key Lab for Thin Film and Micro fabrication of Ministry of Education, State Key Lab of Micro-Nano Fabrication Technology, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University
  • , Shao Jun 邵君Affiliated withDepartment of Bio-Nano Science and Engineering, Key Lab for Thin Film and Micro fabrication of Ministry of Education, State Key Lab of Micro-Nano Fabrication Technology, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University
    • , Bao Chen-chen 鲍晨晨Affiliated withDepartment of Bio-Nano Science and Engineering, Key Lab for Thin Film and Micro fabrication of Ministry of Education, State Key Lab of Micro-Nano Fabrication Technology, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University
    • , Gao Feng 高峰Affiliated withDepartment of Bio-Nano Science and Engineering, Key Lab for Thin Film and Micro fabrication of Ministry of Education, State Key Lab of Micro-Nano Fabrication Technology, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University
    • , He Rong 贺蓉Affiliated withDepartment of Bio-Nano Science and Engineering, Key Lab for Thin Film and Micro fabrication of Ministry of Education, State Key Lab of Micro-Nano Fabrication Technology, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University
    • , Shu Meng-jun 舒孟军Affiliated withDepartment of Bio-Nano Science and Engineering, Key Lab for Thin Film and Micro fabrication of Ministry of Education, State Key Lab of Micro-Nano Fabrication Technology, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University
    • , Ma Yong-jie 马勇杰Affiliated withDepartment of Bio-Nano Science and Engineering, Key Lab for Thin Film and Micro fabrication of Ministry of Education, State Key Lab of Micro-Nano Fabrication Technology, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University

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Abstract

Objective

To investigate the efficiency of polyamidoamine dendrimer grafted carbon nanotube (dendrimer-CNT) mediated entrance of anti-survivin oligonucleotide into MCF-7 cells, and its effects on the growth of MCF-7 cells.

Methods

Antisense survivin oligonucleotide was anchored onto polyamidoamine dendrimer grafted carbon nanotubes to form dendrimer-CNT-asODN complex and the complex was characterized by Zeta potential, AFM, TEM, and 1% agarose gel electrophoresis analysis. Dendrimer-CNT-asODN complexes were added into the medium and incubated with MCF-7 cells. MTT method was used to detect the effects of asODN and dendrimer-CNT-asODN on the growth of MCF-7 cells. TEM was used to observe the distribution of dendrimer-CNT-asODN complex within MCF-7 cells.

Results

Successful synthesis of dendrimer-CNT-asODN complexes was proved by TEM, AFM and agarose gel electrophoresis. TEM showed that the complexes were located in the cytoplasm, endosome, and lysosome within MCF-7 cells. When dendrimer-CNT-asODN (1.0 μmol/L) and asODN (1.0 μmol/L) were used for 120 h incubation, the inhibitory rates of MCF-7 cells were (28.22±3.5)% for dendrimer-CNT-asODN complex group, (9.23±0.56)% for only asODN group, and (3.44±0.25)% for dendrimer-CNT group. Dendrimer-CNT-asODN complex at 3.0 μmol/L inhibited MCF-7 cells by (30.30±10.62)%, and the inhibitory effects were in a time-and concentration-dependent manner.

Conclusion

Dendrimer-CNT nanoparticles may serve as a gene delivery vector with high efficiency, which can bring foreign gene into cancer cells, inhibiting cancer cell proliferation and markedly enhancing the cancer therapy effects.

Key words

Gene delivery Carbon nanotube Polyamidoamine dendrimer Cancer therapy Survivin gene

CLC number

R73-36+2