Chinese Journal of Integrative Medicine

, Volume 19, Issue 8, pp 589–595

Effect of Sanqi Oral Liquid (三芪口服液) on the expressions of CD4+, CD8+ and CD68+ cells in 5/6 nephrectomized rats with chronic renal failure

  • Fang-ning Wei (韦芳宁)
  • Zi-lin Chen (陈子林)
  • Hai-feng Yang (杨海峰)
  • Ling Han (韩 凌)
  • Hai-ming Ding (丁海明)
  • Shi-gui Deng (邓时贵)
  • Run-mei Ou (欧润妹)
  • Ai-hua Ou (欧爱华)
  • Yan-fen Liang (梁燕芬)
  • Zi-heng Hu (胡子衡)
  • Jian Wang (王 健)
  • Xiao-hong Yang (杨小虹)
  • Ni-zhi Yang (杨霓芝)
Original Article

DOI: 10.1007/s11655-012-1233-5

Cite this article as:
Wei, F., Chen, Z., Yang, H. et al. Chin. J. Integr. Med. (2013) 19: 589. doi:10.1007/s11655-012-1233-5

Abstract

Objective

To explore the mechanisms of Chinese herbal medicine Sanqi Oral Liquid (三芪口服液), composed of Astragalus membranaceus and Panpax notoginseng, in alleviating renal injury by observing its effect on the expressions of CD4+, CD8+ and CD68+ cells in 5/6 nephrectomized rats with chronic renal failure.

Methods

A total of 102 SD rats were randomly divided into six groups: three treatment groups were administrated with high, medium and low dosage of Sanqi Oral Liquid respectively by gavage; a normal group, a 5/6 nephrectomized model group, and a group treated with coated aldehyde oxygenstarch were used as controls. Following oral administration of Sanqi Oral Liquid for 12 weeks, the general condition and renal pathological changes were observed, and the renal function, platelet count (PLT) and the expressions of CD4+, CD8+ and CD68+ cells were determined for each group.

Results

There were proliferation of mesangial matrix, renaltubularnecrosis and obvious tubulointerstitial fibrosis in the model group, and they were much milder in the treatment groups. Compared with the model group, the amounts of blood urea nitrogen (BUN), serum creatinine (Scr) and PLT in the treatment groups decreased (P<0.05 for all); and in the group administrated of medium dosage of Sanqi Oral Liquid, the expression of CD4+ cells was up-regulated and those of CD8+ and CD68+ cells were down-regulated (P<0.05 for all), leading to an increased ratio of CD4+/CD8+(P<0.01).

Conclusion

Sanqi Oral Liquid has a significant effect on regulating lymphocyte subsets, reducing the infiltration of macrophages in renal tissues and alleviating tubulointerstitial fibrosis, and this may be one of mechanisms of Sanqi Oral Liquid in delaying the progression of chronic kidney diseases.

Keywords

Sanqi Oral Liquidchronic kidney diseasetubulointerstitial fibrosisAstragalus membranaceusPanpax notoginseng

Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Fang-ning Wei (韦芳宁)
    • 1
  • Zi-lin Chen (陈子林)
    • 2
  • Hai-feng Yang (杨海峰)
    • 3
  • Ling Han (韩 凌)
    • 4
  • Hai-ming Ding (丁海明)
    • 5
  • Shi-gui Deng (邓时贵)
    • 6
  • Run-mei Ou (欧润妹)
    • 6
  • Ai-hua Ou (欧爱华)
    • 7
  • Yan-fen Liang (梁燕芬)
    • 8
  • Zi-heng Hu (胡子衡)
    • 9
  • Jian Wang (王 健)
    • 9
  • Xiao-hong Yang (杨小虹)
    • 9
  • Ni-zhi Yang (杨霓芝)
    • 1
  1. 1.Department of UrologyGuangdong Provincial Hospital of Traditional Chinese MedicineGuangzhouChina
  2. 2.Xinhui Hospital of Traditional Chinese MedicineJiangmen, Guandong ProvinceChina
  3. 3.Department of PathologyGuangdong Provincial Hospital of Traditional Chinese MedicineGuangzhouChina
  4. 4.Guangdong Provincial Scientific Institute of Traditional Chinese MedicineGuangzhouChina
  5. 5.Clinical LaboratoryGuangdong Provincial Hospital of Traditional Chinese MedicineGuangzhouChina
  6. 6.Animal Laboratory CenterGuangdong Provincial Hospital of Traditional Chinese MedicineGuangzhouChina
  7. 7.Epidemiological Teaching and Research SectionGuangdong Provincial Hospital of Traditional Chinese MedicineGuangzhouChina
  8. 8.Pharmaceutical Manufacturing SectionGuangdong Provincial Hospital of Traditional Chinese MedicineGuangzhouChina
  9. 9.Guangzhou University of Chinese MedicineGuangzhouChina