Chinese Journal of Integrative Medicine

, Volume 19, Issue 8, pp 610–615

Comparative study on effects of Xiaoyao Powder (逍遥散) and its modified prescription on plasma metabolomics of rats with chronic immobilization stress

  • He-gu Luo罗和古
  • Jia-xu Chen陈家旭
  • Qi Zhang张 琪
  • Guang-xin Yue岳广欣
  • Jie Ding丁 杰
  • Han-ting Zhang张汉霆
  • Xian-zhong Yan颜贤忠
  • Xin Zhao赵 歆
  • Zhen-zhi Meng蒙缜之
Original Article

DOI: 10.1007/s11655-012-1092-0

Cite this article as:
Luo, H., Chen, J., Zhang, Q. et al. Chin. J. Integr. Med. (2013) 19: 610. doi:10.1007/s11655-012-1092-0

Abstract

Objective

To determine the changes in the levels of endogenous metabolites in rats with chronic immobilization stress (CIS) taking Xiaoyao Powder (逍遥散, XYP) and its modified prescription version, which lacks the volatile oils extracted from Herba Menthae.

Methods

Twenty-four experimental male SD rats were randomly divided into 4 groups of 6 rats each: control, model, XYP-1 (containing volatile oils from Herba Menthae), and XYP-2 (lacking volatile oils). All rats except control group rats were subjected to CIS 3 h per day for 21 consecutive days. Groups XYP-1 and XYP-2 were given the extracted XYS with or without volatile oils (3.854 g/kg; suspended in distilled water) via gavage 1 h before CIS each day for 21 days. Rats were anesthetized using intraperitoneal injection of pentobarbital sodium (40 mg/kg) on the 22nd day. Observations were made using a Varian INOVA 600 MHz NMR spectrometer at 27 °C. Carr-Purcell-Meiboom-Gill (CPMG) and longitudinal eddy-delay (LED) were applied, resulting in spectra showing only the signals from micro- and macro-metabolites.

Results

Compared to controls, rats subjected to CIS showed increased levels of plasma metabolites, such as acetic acid, choline, N-glycoprotein (NAC), saturated fatty acid, and blood sugars. Levels of low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), and unsaturated fatty acids were decreased. The biochemical effects of XYS were characterized by elevated levels of VLDL, LDL, threonine, methionine, and glutamic acid in plasma.

Conclusion

Some common and characteristic metabolites on the anti-CIS of XYP and its modified prescription were obtained. The metabolomics technology is a valuable tool and may be used to identify the specific metabolites and potential biomarkers of therapeutic effect of Chinese medicinal prescriptions.

Keywords

metabolomicschronic immobilization stressbiomarkerChinese medicineXiaoyao Powder

Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • He-gu Luo罗和古
    • 1
  • Jia-xu Chen陈家旭
    • 1
  • Qi Zhang张 琪
    • 2
  • Guang-xin Yue岳广欣
    • 1
  • Jie Ding丁 杰
    • 1
  • Han-ting Zhang张汉霆
    • 3
  • Xian-zhong Yan颜贤忠
    • 2
  • Xin Zhao赵 歆
    • 1
  • Zhen-zhi Meng蒙缜之
    • 1
  1. 1.School of Pre-clinical MedicineBeijing University of Chinese MedicineBeijingChina
  2. 2.National Center of Biomedical AnalysisBeijingChina
  3. 3.Department of Behavioral Medicine and PsychiatryWest Virginia University Health Sciences CenterMorgantownUSA