Chinese Journal of Integrative Medicine

, Volume 15, Issue 5, pp 365–370

Effects of tanshinone II A on the myocardial hypertrophy signal transduction system protein kinase B in rats

  • En-yuan Tu (屠恩远)
  • Ya-guang Zhou (周亚光)
  • Zhao-hua Wang (王照华)
  • Qian-sheng Liang (梁黔生)
  • Guang-tian Yang (杨光田)
Experimental Research

DOI: 10.1007/s11655-009-0365-8

Cite this article as:
Tu, E., Zhou, Y., Wang, Z. et al. Chin. J. Integr. Med. (2009) 15: 365. doi:10.1007/s11655-009-0365-8

Abstract

Objective

To study the effect of tanshinone II A on the cell signal transduction system protein kinase B (Akt) in rats with hypertrophy of the myocardium induced by partial constriction of the thoracic aorta.

Methods

Rat models of myocardial hypertrophy were established by the thoracic aorta partial constriction method. Forty-eight rats were randomly divided into the sham-operative group, the model group, the valsartan treatment group, and the low-, medium-, and high-dose tanshinone treatment groups. The heart mass index (HMI), left ventricular mass index (LVMI), ejection fraction (EF), left ventricular posterior wall (LVPW), and interventricular septal thickness (IVS) were detected by high-frequency ultrasonography. The myocardial fiber diameter (MFD) was detected by HE staining, and the contents of p-Akt and p-Gsk3β in the myocardium were detected by Western blot.

Results

Compared with the sham-operative group, the levels of HMI, LVMI, LVPW, IVS, and MFD were increased respectively in the other groups (P<0.05); the contents of p-Akt and p-Gsk3β were also increased in the other groups. Compared with the model group, the levels of HMI, LVMI, LVPW, IVS, and MFD were decreased respectively in all treatment groups (P<0.05); the contents of p-Akt and p-Gsk3β were decreased in all treatment groups as well. There was no significant difference, however, among the low-, medium-, and high-dose tanshinone treatment groups and the valsartan treatment group (P>0.05).

Conclusion

Tanshinone II A can prevent myocardial hypertrophy by its action on the protein kinase B (Akt) signaling pathway.

Key Words

tanshinone II Amyocardial hypertrophyratprotein kinase Bthoracic aorta coarctation

Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine and Springer Berlin Heidelberg 2009

Authors and Affiliations

  • En-yuan Tu (屠恩远)
    • 1
  • Ya-guang Zhou (周亚光)
    • 1
  • Zhao-hua Wang (王照华)
    • 1
  • Qian-sheng Liang (梁黔生)
    • 1
  • Guang-tian Yang (杨光田)
    • 1
  1. 1.Department of Emergency, Tongji Hospital, Tongji CollegeHuazhong University of Science and TechnologyWuhanChina