Effect of sodium tanshinone II A sulfonate on phosphorylation of extracellular signal-regulated kinase 1/2 in angiotensin II-induced hypertrophy of myocardial cells
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
To observe the effects of sodium tanshinone II A sulfonate (STS) on angiotensin II (Ang II)-induced hypertrophy of myocardial cells through the expression of phosphorylated extracellular signal-regulated kinase (p-ERK1/2).
In the primary culture of neonatal rat myocardial cells, the total protein content in myocardial cells was determined by coomassie brilliant blue and the protein synthesis rate was measured by [3H]-Leucine incorporation as indexes for hypertrophy of myocardial cells. The expression of p-ERK1/2 was determined using Western blot and immunofluorescence labeling.
(1) The total protein and protein synthesis rate increased significantly in contrast to the control group after the myocardial cells were stimulated by Ang II (1 μmol/L) for 24 h; STS markedly inhibited the increment of the total protein level induced by Ang II and the syntheses of protein. (2) After pretreatment of myocardial cells with Ang II (1 μmol/L) for 5 min, the p-ERK1/2 protein expression was increased, with the most obvious effect shown at about 10 min; pretreatment of myocardial cells with STS at different doses (2, 10, 50 μmol/L) for 30 min resulted in obvious inhibition of the expression of p-ERK1/2 stimulated by Ang II in a dose-dependent manner. (3) After the myocardial cells were stimulated by Ang II (1 μmol/L), the immunofl uorescence of ERK1/2 rapidly appeared in the nucleus. The activation and translocation process of ERK1/2 induced by Ang II was blocked distinctly by STS.
STS inhibited the myocardial cell hypertrophy induced by Ang II, and the mechanism may be associated with the inhibition of p-ERK1/2 expression.
- Cohn, J.N., Ferrari, R., Sharpe, N. (2000) Cardiac remodeling-concepts and clinical implications: a consensus paper from an international forum on cardiac remodeling. Behalf on an international forum on cardiac remodeling. J Am Coll Cardiol 35: pp. 569-582 CrossRef
- Liu, F., Zheng, Z. (2004) The mechanisms of radix salviae miltiorrhizae in prevention of left ventricular hypertrophy in spontaneously hypertensive rats. Chin J Emerg Med (Chin) 13: pp. 322-324
- Kesteren, C.A., Heugten, H.A., Lamers, J.M. (1997) Angiotensin II-mediated growth and antigrowth effects in cultured neonatal rat cardiac myocytes and fibroblasts. J Mol Cell Cardiol 29: pp. 2147-2157 CrossRef
- Yamazaki, T., Komuro, I., Kudoh, S. (1995) Angiotensin II partly mediates mechanical stress-induced cardiac hypertrophy. Circ Res 77: pp. 258-265
- Frey, N., Olson, E.N. (2003) Cardiac hypertrophy: the good, the bad and the ugly. Annu Rev Physiol 65: pp. 45-79 CrossRef
- Frey, N., Katus, H.A., Olson, E.N. (2004) Hypertrophy of the heart: a new therapeutic target?. Circulation 109: pp. 1580-1589 CrossRef
- Sugden, P.H. (2001) Signalling pathways in cardiac myocyte hypertrophy. Ann Med 33: pp. 611-622
- Zhang, S.Q., Ding, B., Guo, Z.G. (2004) Inhibitory effect of antisense oligodeoxynucleotide to 44/p42 MAPK on angiotensin II-induced hypertrophic response in cultured neonatal rat cardiac myocyte. Acta Pharmacol Sin 25: pp. 41-46
- Ouyang, X., Takahashi, K., Komatsu, K. (2001) Protective effect of Salvia miltiorrhiza on angiotensin II-induced hypertrophic responses in neonatal rat cardiac cells. Jpn J Pharmacol 87: pp. 289-96 CrossRef
- Xie, H., Zheng, Z. (2004) The effect of tanshinon II A on hypertrophy and apoptosis of cardiomycytes induced by angiotensin II. Chin J Hypertens (Chin) 12: pp. 359-361
- Effect of sodium tanshinone II A sulfonate on phosphorylation of extracellular signal-regulated kinase 1/2 in angiotensin II-induced hypertrophy of myocardial cells
Chinese Journal of Integrative Medicine
Volume 14, Issue 2 , pp 123-127
- Cover Date
- Print ISSN
- Online ISSN
- Chinese Association of Traditional and Western Medicine
- Additional Links
- sodium tanshinone II A sulfonate
- angiotensin II
- myocardial cell hypertrophy
- extracellular signal-regulated kinase
- Industry Sectors