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Expression of dehydrins, HSP70, Cu/Zn SOD, and RuBisCO in leaves of tobacco (Nicotiana tabacum L.) dihaploids under salt stress

  • Abiotic Stress Responses
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Abstract

Salinity is an abiotic factor that has a negative impact on plant growth and seed germination, leading to limited crop production. The effect of salt stress (100 and 200 mM NaCl) on the expression of dehydrins (DHNs), heat-shock protein 70 (HSP70), superoxide dismutase (Cu/Zn SOD), and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) was investigated in leaves of tobacco (Nicotiana tabacum L.) F1 hybrid DH10 and four dihaploid lines (207B, 238C, 239K, 244B) under in vitro conditions. After 33 d, the dihaploid lines as well as DH10 showed enhanced expression of 70-kDa DHN in response to 200 mM NaCl stress. This was especially evident in line 207B, which previously proved to be tolerant to salt stress, suggesting that DHNs have an important role in salt stress tolerance. Also, line 207B as well as lines 238C and 244B responded to 200 mM NaCl stress by increased expression of Cu/Zn SOD, indicating its possible role in providing salt stress tolerance. On the other hand, salt stress induced in line 239K resulted in a significantly higher expression of RuBisCO, indicating that differential susceptibility of dihaploids to increased levels of NaCl might be the result of their different genetic traits. Decreased expression of HSP70 in salt-treated tobacco dihaploids indicates that HSP70 is not included in protection against salt stress.

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Acknowledgment

The authors thank Dr. Timothy Close from the Department of Botany and Plant Sciences, University of California, for providing the dehydrin antibody.

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Authors and Affiliations

  1. Subdepartment of Biology and Microbiology, Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, HR-31000, Osijek, Croatia

    Tihana Marček

  2. Department of Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, HR-10000, Zagreb, Croatia

    Mirta Tkalec, Željka Vidaković-Cifrek, Marin Ježić & Mirna Ćurković-Perica

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  1. Tihana Marček
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  2. Mirta Tkalec
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  3. Željka Vidaković-Cifrek
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  4. Marin Ježić
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  5. Mirna Ćurković-Perica
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Correspondence to Mirna Ćurković-Perica.

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Editor: Kazuo Watanabe

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Marček, T., Tkalec, M., Vidaković-Cifrek, Ž. et al. Expression of dehydrins, HSP70, Cu/Zn SOD, and RuBisCO in leaves of tobacco (Nicotiana tabacum L.) dihaploids under salt stress. In Vitro Cell.Dev.Biol.-Plant 52, 233–240 (2016). https://doi.org/10.1007/s11627-016-9752-y

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  • DOI: https://doi.org/10.1007/s11627-016-9752-y

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