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Histochemical evaluation of induction of somatic embryogenesis in Passiflora edulis Sims (Passifloraceae)

  • Plant Tissue Culture
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Abstract

The aim of this study was to describe the accumulation of reserves during the somatic embryogenesis process in Passiflora edulis Sims FB-300, obtained from mature zygotic embryos, using histochemical methods. Mature zygotic embryos were inoculated in Murashige and Skoog induction media supplemented with 31.06 μM of picloram, 2.22 μM of benzyladenine, and 2.27 μM of thidiazuron. The zygotic embryo explants, at different developmental stages, were collected and fixed in Karnovsky solution and subsequently subjected to dehydration in an ethanol series and embedded in acrylic resin. Transverse and longitudinal sections (5-μm thick) were stained with toluidine blue for anatomical characterization, using Xylidine Ponceau for the detection of total protein, periodic acid-Schiff reagent for neutral polysaccharides, Sudan black B to detect lipids, and Lugol’s reagent for the starch detection. Histological sections revealed the formation of protuberances and globular stage somatic embryos in the cotyledonary region of the zygotic embryo. Histochemical tests revealed the presence of large quantities of protein bodies in zygotic embryos of P. edulis, which were gradually metabolized during somatic embryo development. Lipid bodies and starch grains were identified only after 20 d of culture, suggesting the use of these compounds as energy source for de novo synthesis. The present study describes the anatomical changes and the pattern of reserve accumulation during the somatic embryogenesis process in P. edulis.

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Acknowledgments

The authors would like to thank the CAPES for the financial support, the Viveiros Flora Brasil Ltda. (Araguari, MG) for generously providing the Maguary seeds of the Araguari ‘FB-300’ population, and the NMM-UFV (Viçosa, MG) for the use of the electron microscope.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Genética e Melhoramento de Plantas, Universidade do Estado de Mato Grosso, Rod. MT 358, Km 7, Jardim Aeroporto, Tangará da Serra, Mato Grosso, 78300000, Brazil

    Gizelly M. da Silva

  2. Departamento de Biologia Vegetal, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Viçosa, Av. Peter Henry Rolfs, s/n, Viçosa, Minas Gerais, 36570000, Brazil

    Ana C. F. da Cruz & Wagner C. Otoni

  3. Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. Alberto Lamego, 2000, Horto, Campos dos Goytacazes, Rio de Janeiro, 28013602, Brazil

    Telma N. S. Pereira

  4. Departamento de Fisiologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, Monteiro Lobato, 255, Cidade Universitária Zeferino, Campinas, São Paulo, 13083862, Brazil

    Diego I. Rocha

  5. Departamento de Biologia, Universidade do Estado de Mato Grosso, Rod. MT 358, Km 7, Jardim Aeroporto, Tangará da Serra, Mato Grosso, 78300000, Brazil

    Maurecilne L. da Silva

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  1. Gizelly M. da Silva
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  2. Ana C. F. da Cruz
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  3. Wagner C. Otoni
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  4. Telma N. S. Pereira
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  5. Diego I. Rocha
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  6. Maurecilne L. da Silva
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Correspondence to Maurecilne L. da Silva.

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Editor: Ewen Mullins

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da Silva, G.M., da Cruz, A.C.F., Otoni, W.C. et al. Histochemical evaluation of induction of somatic embryogenesis in Passiflora edulis Sims (Passifloraceae). In Vitro Cell.Dev.Biol.-Plant 51, 539–545 (2015). https://doi.org/10.1007/s11627-015-9699-4

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  • DOI: https://doi.org/10.1007/s11627-015-9699-4

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