In vitro propagation of Artocarpus altilis (park.) fosberg (breadfruit) from mature plant material

Article

DOI: 10.1007/s11627-000-0023-5

Cite this article as:
Rouse-Miller, J. & Duncan, J.E. In Vitro Cell.Dev.Biol.-Plant (2000) 36: 115. doi:10.1007/s11627-000-0023-5

Summary

A micropropagation protocol for Artocarpus altilis (breadfruit, yellow cultivar) using shoot tip explants taken from mature trees is outlined. Cultures were initiated from shoot tip explants (1–2 mm in length) on either N5K or N15K (Margara, 1978) macronutrient formulation, MS (Murashige and Skoog, 1962) micronutrients and vitamins and 6-benzyladenine (BA, 4.4 μM). Single-node explants obtained from shoots formed at the initiation stage were used at the multiplication stage. Multiplication elongation and maintenance were possible on N30NH4 (Margara, 1978) macronutrients, MS micronutrients and vitamins and zeatin 2.2 μM. Shoot elongation was not enhanced by the inclusion of gibberelic acid at 1.4 μM, 2.8 μM or 14.0 μM, along with zeatin at 2.2 μM, when compared with those shoots cultured on zeatin only. N30NH4 was found to be superior (with respect to shoot quality) to MS macronutrient formulations at the multiplication and maintenance stages, because the shoots with development were more vigorous than those formed on the other macronutrient formulations mentioned. Zeatin at 2.2 μM yielded 50% more in vitro-formed shoots when compared with BA at 4.4 μM for the same time period. Shoots rooted on hormone-free medium with a success rate of 60%. Regenerated plantlets were hardened with about a 40% success rate.

Key words

media micropropagation apical buds Artocarpus tree species 

Copyright information

© Society for In Vitro Biology 2000

Authors and Affiliations

  1. 1.Department of Life SciencesThe University of the West IndiesSt AngustineTrinidad and Tobago, West Indies

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