In vitro regeneration of Echinacea purpurea L.: Direct somatic embryogenesis and indirect shoot organogenesis in petiole culture
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
An in vitro propagation system was developed for Echinacea purpurea L. (purple coneflower), a medicinal plant commonly used in the treatment of colds, flu and related ailments. Echinacea seeds were found to be contaminated with systemic fungi and therefore an optimized minimal concentration of Plant Preservation Mixture (PPM) was incorporated in the seed germination medium to recover sterile seedlings. Regeneration was induced on petiole explants from 2-month-old sterile seedlings cultured on medium supplemented with benzylaminopurine (BAP) or thidiazuron (TDZ) in combination with indoleacetic acid (IAA). Two distinct forms of regeneration were identified in cultured petiole explants with histological and morphological observations, viz. the direct formation of somatic embryos on the epidermis and the de novo development of shoots from callus tissues formed in subepidermal cell layers. the results of this study have established a micropropagation system for E. purpurea that will provide sterile plant material for further investigations into medicinally active biochemicals and may facilitate mass production of high-quality E. purpurea plants for the commercial market.
- Coeugniet, E. G.; Elek, E. Immunomodulation with Viscum album and Echinacea purpurea extracts. Onkologie, 27–33; June, 1987.
- Facino, R. M.; Carini, M.; Aldini, G.; Saibene, L.; Pietta, P.; Mauri, P. Echinacoside and caffeoyl conjugates protect collagen from free radical-induced degradation: a potential use of Echinacea extracts in the prevention of skin photodamage. Planta Med. 61:510–514; 1995. CrossRef
- Gamborg, O. L.; Miller, R. A.; Ojima, K. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res. 50:151–158; 1968. CrossRef
- Huetteman, C. A.; Preece, J. E. Ihidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss. Org. Cult. 33:105–119; 1993. CrossRef
- Jensen, W. A. Botanical histochemistry. San Francisco: W.H. Freeman; 1962.
- Kindscher, K. Medicinal wild plants of the prairie lawrence. University Press of Kansas; 1992;86.
- Li, T. S. C. Echinacea: cultivation and medicinal value. Hort Tech. 8:122–129; 1998.
- Lu, C-Y. The use of thidiazuron in tissue culture. In Vitro Cell. Dev. Biol.-Plant 29:92–96; 1993.
- Mengs, U.; Clare, C. B.; Poiley, J. A., Toxicity of Echinacea purpurea. Arzneim.-Forsch/Drug Res. 41 (11):1076–1081; 1991.
- Murashige, T.; Skoog, F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962. CrossRef
- Murthy, B. N. S.; Murch, S. J.; Saxena, P. K. Thidiazuron-induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogaea L.): endogenous growth regulator levels and significance of cotyledons. Physiol. Plant 94:268–276; 1995. CrossRef
- Murthy, B. N. S.; Victor, J.; Singh, R. P.; Fletcher, R. A.; Saxena, P. K. In vitro regeneration of chickpea (Cicer arietinum L.): Stimulation of direct organogenesis and somatic embryogenesis by thidiazuron. Plant Growth Reg. 19:233–240; 1996. CrossRef
- Murthy, B. N. S.; Murch, S. J.; Saxena, P. K. Thidiazuron: A potent regulator of in vitro plant morphogenesis. In Vitro Cell. Dev. Biol.-Plant 34:267–275; 1998.
- SAS Institute Statistical analysis systems users' guide: statistics version 6. Cary, NC: SAS Institute; 1995.
- Schardt, D. Still out in the cold. Nutrition Action Health Letter. April, 1998.
- Sharp, W. R.; Sondhal, M. R.; Caldar, R. S.; Maraffa, S. B. The physiology of in vitro asexual embryogenesis. Hortic. Rev. 2:268–310; 1986.
- Skoog, F.; Miller, C. O. Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp. Soc. Exp. Biol. 11:118–140; 1957.
- Steinmüller, C.; Roesler, J.; Gröttrup, E.; Franke, G.; Wagner, H.; Lohmann-Matthes, M. L. Polysaccharides isolated from plant cell cultures of Echinacea purpurea enhance the resistance of immunosuppressed mice against systemic infections with Candida albicans and Listeria monocytogenes. Int. J. Immunopharmacol. 15:605–614; 1993. CrossRef
- Steward, F. C.; Mapes, M. O.; Kent, A. E.; Holsten, R. D. Growth and development of cultured plant cells. Biochemical and morphogenic studies with cells yield new evidence on their metabolism and totipotency. Science 143:20–27; 1964. CrossRef
- Stimpel, M.; Proksch, A.; Wagner, H.; Lohmann-Matthes, M.-L. Macrophage activation and induction of macrophage cytotoxicity by purified polysaccharide fractions from the plant Echinacea purpurea. Infect. Immun. 46(3):845–849; 1984.
- Tragni, E.; Galli, C. L.; Tubaro, A.; Del Negro, P.; Della Loggia, R. Anti-inflammatory activity of Echinacea angustifolia fractions separated on the basis of molecular weight. Pharm. Res. Commun. 20(suppl): 87–90; 1988.
- Visser, C.; Qureshi, J. A.; Gill, R.; Malik, K. A.; Saxena, P. K. Morphoregulatory role of thidiazuron: Substitution of auxin and cytokinin requirement for the induction of somatic embryogenesis in geranium hypocotyl cultures. Plant Physiol. 99:1704–1707; 1992. CrossRef
- In vitro regeneration of Echinacea purpurea L.: Direct somatic embryogenesis and indirect shoot organogenesis in petiole culture
In Vitro Cellular & Developmental Biology - Plant
Volume 36, Issue 1 , pp 30-36
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Echinacea purpurea L
- Industry Sectors