Article

In Vitro Cellular & Developmental Biology - Animal

, Volume 49, Issue 2, pp 147-154

First online:

Isolation, characterization, and mesodermic differentiation of stem cells from adipose tissue of camel (Camelus dromedarius)

  • Abdollah Mohammadi-SangcheshmehAffiliated withStem Cell Biology Department, Stem Cell Technology Research CenterDepartment of Animal and Poultry Science, College of Aburaihan, University of Tehran
  • , Abbas ShafieeAffiliated withStem Cell Biology Department, Stem Cell Technology Research Center
  • , Ehsan SeyedjafariAffiliated withStem Cell Biology Department, Stem Cell Technology Research CenterDepartment of Biotechnology, College of Science, University of Tehran
  • , Peyman DinarvandAffiliated withStem Cell Biology Department, Stem Cell Technology Research Center
  • , Abdolhakim ToghdoryAffiliated withDepartment of Animal Science, Gorgan University of Agricultural Science and Natural Resources
  • , Iman BagherizadehAffiliated withStem Cell Biology Department, Stem Cell Technology Research Center
  • , Karl SchellanderAffiliated withInstitute of Animal Science, University of Bonn
  • , Mehmet Ulas CinarAffiliated withInstitute of Animal Science, University of Bonn
  • , Masoud SoleimaniAffiliated withStem Cell Biology Department, Stem Cell Technology Research CenterDepartment of Hematology, Faculty of Medical Science, Tarbiat Modares University Email author 

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Abstract

Adipose-derived stem cells are an attractive alternative as a source of stem cells that can easily be extracted from adipose tissue. Isolation, characterization, and multi-lineage differentiation of adipose-derived stem cells have been described for human and a number of other species. Here we aimed to isolate and characterize camel adipose-derived stromal cell frequency and growth characteristics and assess their adipogenic, osteogenic, and chondrogenic differentiation potential. Samples were obtained from five adult dromedary camels. Fat from abdominal deposits were obtained from each camel and adipose-derived stem cells were isolated by enzymatic digestion as previously reported elsewhere for adipose tissue. Cultures were kept until confluency and subsequently were subjected to differentiation protocols to evaluate adipogenic, osteogenic, and chondrogenic potential. The morphology of resultant camel adipose-derived stem cells appeared to be spindle-shaped fibroblastic morphology, and these cells retained their biological properties during in vitro expansion with no sign of abnormality in karyotype. Under inductive conditions, primary adipose-derived stem cells maintained their lineage differentiation potential into adipogenic, osteogenic, and chondrogenic lineages during subsequent passages. Our observation showed that like human lipoaspirate, camel adipose tissue also contain multi-potent cells and may represent an important stem cell source both for veterinary cell therapy and preclinical studies as well.

Keywords

Adipose tissue Stem cell Adipogenic Osteogenic Chondrogenic Camelus dromedarius