In Vitro Cellular & Developmental Biology - Animal

, Volume 49, Issue 2, pp 147–154

Isolation, characterization, and mesodermic differentiation of stem cells from adipose tissue of camel (Camelus dromedarius)

  • Abdollah Mohammadi-Sangcheshmeh
  • Abbas Shafiee
  • Ehsan Seyedjafari
  • Peyman Dinarvand
  • Abdolhakim Toghdory
  • Iman Bagherizadeh
  • Karl Schellander
  • Mehmet Ulas Cinar
  • Masoud Soleimani
Article

DOI: 10.1007/s11626-012-9578-9

Cite this article as:
Mohammadi-Sangcheshmeh, A., Shafiee, A., Seyedjafari, E. et al. In Vitro Cell.Dev.Biol.-Animal (2013) 49: 147. doi:10.1007/s11626-012-9578-9

Abstract

Adipose-derived stem cells are an attractive alternative as a source of stem cells that can easily be extracted from adipose tissue. Isolation, characterization, and multi-lineage differentiation of adipose-derived stem cells have been described for human and a number of other species. Here we aimed to isolate and characterize camel adipose-derived stromal cell frequency and growth characteristics and assess their adipogenic, osteogenic, and chondrogenic differentiation potential. Samples were obtained from five adult dromedary camels. Fat from abdominal deposits were obtained from each camel and adipose-derived stem cells were isolated by enzymatic digestion as previously reported elsewhere for adipose tissue. Cultures were kept until confluency and subsequently were subjected to differentiation protocols to evaluate adipogenic, osteogenic, and chondrogenic potential. The morphology of resultant camel adipose-derived stem cells appeared to be spindle-shaped fibroblastic morphology, and these cells retained their biological properties during in vitro expansion with no sign of abnormality in karyotype. Under inductive conditions, primary adipose-derived stem cells maintained their lineage differentiation potential into adipogenic, osteogenic, and chondrogenic lineages during subsequent passages. Our observation showed that like human lipoaspirate, camel adipose tissue also contain multi-potent cells and may represent an important stem cell source both for veterinary cell therapy and preclinical studies as well.

Keywords

Adipose tissueStem cellAdipogenicOsteogenicChondrogenicCamelus dromedarius

Copyright information

© The Society for In Vitro Biology 2013

Authors and Affiliations

  • Abdollah Mohammadi-Sangcheshmeh
    • 1
    • 2
  • Abbas Shafiee
    • 1
  • Ehsan Seyedjafari
    • 1
    • 3
  • Peyman Dinarvand
    • 1
  • Abdolhakim Toghdory
    • 4
  • Iman Bagherizadeh
    • 1
  • Karl Schellander
    • 5
  • Mehmet Ulas Cinar
    • 5
  • Masoud Soleimani
    • 1
    • 6
  1. 1.Stem Cell Biology DepartmentStem Cell Technology Research CenterTehranIran
  2. 2.Department of Animal and Poultry Science, College of AburaihanUniversity of TehranPakdashtIran
  3. 3.Department of Biotechnology, College of ScienceUniversity of TehranTehranIran
  4. 4.Department of Animal ScienceGorgan University of Agricultural Science and Natural ResourcesGorganIran
  5. 5.Institute of Animal ScienceUniversity of BonnBonnGermany
  6. 6.Department of Hematology, Faculty of Medical ScienceTarbiat Modares UniversityTehranIran