Efficient establishment of primary fibroblast cultures from the hawksbill sea turtle (Eretmochelys imbricata)
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The hawksbill sea turtle (Eretmochelys imbricata) is a critically endangered species at a risk of extinction. Preservation of the genomic and cellular information of endangered animals is important for future genetic and biological studies. Here, we report the efficient establishment of primary fibroblast cultures from skin tissue of the hawksbill sea turtle. We succeeded in establishing 19 primary cultures from 20 hawksbill sea turtle individuals (a success rate of 95%). These cells exhibited a fibroblast-like morphology and grew optimally at a temperature of 26°C, but experienced a loss of viability when cultured at 37°C. Chromosomal analysis using the primary cells derived here revealed that hawksbill sea turtles have a 2n = 56 karyotype. Furthermore, we showed that our primary cell cultures are free of several fish-related viruses, and this finding is important for preservation purposes. To our knowledge, this report is the first to describe primary cell cultures established from normal tissues of the hawksbill sea turtle. The results will contribute to the preservation of biodiversity, especially for the sea turtles that are critically endangered owing to human activities.
- Associated Press. San Diego’s Frozen Zoo. 2009 http://www.cbsnews.com/stories/2002/10/14/tech/main525521.shtml.
- Bass A. L.; Good D. A.; Bjorndal K. A.; Richardson J. I.; Hillis Z. M.; Horrocks J. A.; Bowen B. W. Testing models of female reproductive migratory behaviour and population structure in the Caribbean hawksbill turtle, Eretmochelys imbricata, with mtDNA sequences. Mol. Ecol. 5(3): 321–328; 1996.
- Ben-Nun I. F.; Montague S. C.; Houck M. L.; Tran H. T.; Garitaonandia I.; Leonardo T. R.; Wang Y. C.; Charter S. J.; Laurent L. C.; Ryder O. A.; Loring J. F. Induced pluripotent stem cells from highly endangered species. Nat. Methods 8(10): 829–831; 2011. doi:10.1038/nmeth.1706. CrossRef
- Flindt R. Amazing numbers in biology. 6th ed. Springer, Ludwingsburg; 2003.
- Groombridge B. The IUCN Amphibia–Reptilia Red Data Book Part I. International Union for the Conservation of Nature, Gland; 1982.
- Lu Y.; Nerurkar V. R.; Aguirre A. A.; Work T. M.; Balazs G. H.; Yanagihara R. Establishment and characterization of 13 cell lines from a green turtle (Chelonia mydas) with fibropapillomas. In Vitro Cell. Dev. Biol. Anim. 35(7): 389–393; 1999. doi:10.1007/s11626-999-0113-6. CrossRef
- Moore M. K.; Work T. M.; Balazs G. H.; Docherty D. E. Preparation, cryopreservation, and growth of cells prepared from the green turtle (Chelonia mydas). Methods Cell Sci. 19(3): 161–168; 1997. CrossRef
- Takahashi K.; Tanabe K.; Ohnuki M.; Narita M.; Ichisaka T.; Tomoda K.; Yamanaka S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131(5): 861–872; 2007. doi:10.1016/j.cell.2007.11.019. CrossRef
- Efficient establishment of primary fibroblast cultures from the hawksbill sea turtle (Eretmochelys imbricata)
In Vitro Cellular & Developmental Biology - Animal
Volume 48, Issue 10 , pp 660-665
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- Hawksbill sea turtle
- Cultured cells
- Critically endangered animals
- Chromosome analysis
- Industry Sectors
- Author Affiliations
- 1. Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi Aobaku, Sendai, 981-8555, Japan
- 2. National Research Institute of Aquaculture, Fisheries Research Agency of Japan, Watarainakatuhamaura, Mie, Japan
- 3. Usa Marine Biological Institute, Kochi University, Kami, Kochi, Japan
- 4. Port of Nagoya Public Aquarium, Nagoya, Aichi, Japan
- 5. Ecological Genetics Analysis Section, Center for Environmental Biology and Ecosystem, National Institute for Environmental Studies of Japan, Tsukuba, Japan
- 6. Health Risk Research Section, Center for Environmental Risk Research, National Institute for Environmental Studies, Tsukuba, Japan
- 7. Wildlife Research Center of Kyoto University, Kyoto, Japan