Efficient establishment of primary fibroblast cultures from the hawksbill sea turtle (Eretmochelys imbricata)
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The hawksbill sea turtle (Eretmochelys imbricata) is a critically endangered species at a risk of extinction. Preservation of the genomic and cellular information of endangered animals is important for future genetic and biological studies. Here, we report the efficient establishment of primary fibroblast cultures from skin tissue of the hawksbill sea turtle. We succeeded in establishing 19 primary cultures from 20 hawksbill sea turtle individuals (a success rate of 95%). These cells exhibited a fibroblast-like morphology and grew optimally at a temperature of 26°C, but experienced a loss of viability when cultured at 37°C. Chromosomal analysis using the primary cells derived here revealed that hawksbill sea turtles have a 2n = 56 karyotype. Furthermore, we showed that our primary cell cultures are free of several fish-related viruses, and this finding is important for preservation purposes. To our knowledge, this report is the first to describe primary cell cultures established from normal tissues of the hawksbill sea turtle. The results will contribute to the preservation of biodiversity, especially for the sea turtles that are critically endangered owing to human activities.
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- Efficient establishment of primary fibroblast cultures from the hawksbill sea turtle (Eretmochelys imbricata)
In Vitro Cellular & Developmental Biology - Animal
Volume 48, Issue 10 , pp 660-665
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- Hawksbill sea turtle
- Cultured cells
- Critically endangered animals
- Chromosome analysis
- Industry Sectors
- Author Affiliations
- 1. Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi Aobaku, Sendai, 981-8555, Japan
- 2. National Research Institute of Aquaculture, Fisheries Research Agency of Japan, Watarainakatuhamaura, Mie, Japan
- 3. Usa Marine Biological Institute, Kochi University, Kami, Kochi, Japan
- 4. Port of Nagoya Public Aquarium, Nagoya, Aichi, Japan
- 5. Ecological Genetics Analysis Section, Center for Environmental Biology and Ecosystem, National Institute for Environmental Studies of Japan, Tsukuba, Japan
- 6. Health Risk Research Section, Center for Environmental Risk Research, National Institute for Environmental Studies, Tsukuba, Japan
- 7. Wildlife Research Center of Kyoto University, Kyoto, Japan