Inhibitory effects of Carpinus tschonoskii leaves extract on CpG-stimulated pro-inflammatory cytokine production in murine bone marrow-derived macrophages and dendritic cells

  • Jung-Eun Koo
  • Hye-Jin Hong
  • Vivek Bhakta Mathema
  • Hee-Kyoung Kang
  • Jin-Won Hyun
  • Gi-Young Kim
  • Young-Ree Kim
  • Young-Hee Maeng
  • Chang-Lim Hyun
  • Weon-Young Chang
  • Young-Sang Koh
Report

DOI: 10.1007/s11626-012-9495-y

Cite this article as:
Koo, JE., Hong, HJ., Mathema, V.B. et al. In Vitro Cell.Dev.Biol.-Animal (2012) 48: 197. doi:10.1007/s11626-012-9495-y

Abstract

This report describes the anti-inflammatory effects of MeOH extract from leaves of Carpinus tschonoskii (CE) on primary bone marrow-derived macrophage (BMDMs) and dendritic cells (BMDCs). Primary BMDMs and BMDCs were used for pro-inflammatory cytokine production and Western blot analysis. Human embryonic kidney cell line 293 T (HEK293 T) was used to access NF-κB activity. In all cases, CpG DNA was used to stimulate the cells. The CE (0–150 μg/ml) was treated to BMDMs, BMDCs, and HEK293T cells. CE pre-treatment in CpG-stimulated BMDMs and BMDCs showed a dose-dependent inhibitory effect on pro-inflammatory cytokine (e.g., IL-12 p40, IL-6, and TNF-α) production as compared to non-treated controls. The CE pre-treatment had no significant inhibition on mitogen-activated protein kinases (MAPKs) phosphorylation but strongly inhibited IκBα degradation. In NF-κB reporter gene assay, the CE pre-treatment inhibited NF-κB-dependent luciferase activity in a dose-dependent manner. Taken together, these data suggest that CE has significant inhibitory effect on pro-inflammatory cytokine production and warrant further studies concerning potentials of CE for medicinal uses.

Keywords

Carpinus tschonoskii Interleukin Inflammation NF-κB 

Copyright information

© The Society for In Vitro Biology 2012

Authors and Affiliations

  • Jung-Eun Koo
    • 1
    • 2
  • Hye-Jin Hong
    • 1
    • 2
  • Vivek Bhakta Mathema
    • 1
    • 2
  • Hee-Kyoung Kang
    • 1
    • 2
  • Jin-Won Hyun
    • 1
    • 2
  • Gi-Young Kim
    • 3
  • Young-Ree Kim
    • 1
    • 2
  • Young-Hee Maeng
    • 1
    • 2
  • Chang-Lim Hyun
    • 1
  • Weon-Young Chang
    • 1
  • Young-Sang Koh
    • 1
    • 2
    • 4
  1. 1.School of MedicineJeju National UniversityJejuSouth Korea
  2. 2.Institute of Medical ScienceJeju National UniversityJejuSouth Korea
  3. 3.Department of Marine Life SciencesJeju National UniversityJejuSouth Korea
  4. 4.Department of Microbiology and Immunology, School of MedicineJeju National UniversityJejuSouth Korea