Electrospun PLGA–silk fibroin–collagen nanofibrous scaffolds for nerve tissue engineering

  • Guanglin Wang
  • Xudong Hu
  • Wei Lin
  • Changchao Dong
  • Hui Wu
Article

DOI: 10.1007/s11626-010-9381-4

Cite this article as:
Wang, G., Hu, X., Lin, W. et al. In Vitro Cell.Dev.Biol.-Animal (2011) 47: 234. doi:10.1007/s11626-010-9381-4

Abstract

Electrospun nanofibrous scaffolds varying different materials are fabricated for tissue engineering. PLGA, silk fibroin, and collagen-derived scaffolds have been proved on good biocompatibility with neurons. However, no systematic studies have been performed to examine the PLGA–silk fibroin–collagen (PLGA-SF-COL) biocomposite fiber matrices for nerve tissue engineering. In this study, different weight ratio PLGA-SF-COL (50:25:25, 30:35:35) scaffolds were produced via electrospinning. The physical and mechanical properties were tested. The average fiber diameter ranged from 280 + 26 to 168 + 21 nm with high porosity and hydrophilicity; the tensile strength was 1.76 ± 0.32 and 1.25 ± 0.20 Mpa, respectively. The results demonstrated that electrospinning polymer blending is a simple and effective approach for fabricating novel biocomposite nanofibrous scaffolds. The properties of the scaffolds can be strongly influenced by the concentration of collagen and silk fibroin in the biocomposite. To assay the cytocompatibility, Schwann cells were seeded on the scaffolds; cell attachment, growth morphology, and proliferation were studied. SEM and MTT results confirmed that PLGA-SF-COL scaffolds particularly the one that contains 50% PLGA, 25% silk fibroin, and 25% collagen is more suitable for nerve tissue engineering compared to PLGA nanofibrous scaffolds.

Keywords

Electrospun PLGA Silk fibroin Collagen Schwann Nerve tissue engineering 

Copyright information

© The Society for In Vitro Biology 2010

Authors and Affiliations

  • Guanglin Wang
    • 1
  • Xudong Hu
    • 1
  • Wei Lin
    • 2
  • Changchao Dong
    • 1
  • Hui Wu
    • 1
  1. 1.Department of Orthopaedics, West China HospitalSichuan UniversityChengduPeople’s Republic of China
  2. 2.Department of Gynecology and Obstetrics, West China Second HospitalSichuan UniversityChengduPeople’s Republic of China

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