In Vitro Cellular & Developmental Biology - Animal

, Volume 47, Issue 2, pp 104–113

In vitro growth of microsporidia Anncaliia algerae in cell lines from warm water fish

  • S. Richelle Monaghan
  • Rebecca L. Rumney
  • Nguyen T. K. Vo
  • Niels C. Bols
  • Lucy E. J. Lee
Article

DOI: 10.1007/s11626-010-9366-3

Cite this article as:
Monaghan, S.R., Rumney, R.L., Vo, N.T.K. et al. In Vitro Cell.Dev.Biol.-Animal (2011) 47: 104. doi:10.1007/s11626-010-9366-3

Abstract

Anncaliia algerae is an aquatic microsporidium that most commonly infects mosquitoes but can be grown on the rabbit kidney cell line, RK-13. Spores were purified from RK-13 cultures and added to cell lines from warm water fish and from an insect. The cell lines were GFSK-S1 and GFB3C-W1 from goldfish skin and brain respectively, ZEB2J from zebrafish embryos, FHMT-W1 from fathead minnow testis, and Sf9 from ovaries of a fall armyworm moth. All cultures were maintained at 27°C. Infection was judged to have taken place by the appearance of sporonts and/or spores in cells and occurred in all cell lines. Spores were also isolated from ZEB2J cultures and used to successfully infect new cultures of ZEB2J, RK-13 and Sf9. These results suggest that cells of a wide range of vertebrates support A. algerae growth in vitro and fish cells can produce spores infectious to cells of mammals, fish, and insects

Keywords

MicrosporidiaCell linesGoldfishZebrafishFathead minnowAnncaliia algeraeBrachiolaNosemaRK-13Sf9

Copyright information

© The Society for In Vitro Biology 2010

Authors and Affiliations

  • S. Richelle Monaghan
    • 1
    • 2
  • Rebecca L. Rumney
    • 2
  • Nguyen T. K. Vo
    • 2
  • Niels C. Bols
    • 1
  • Lucy E. J. Lee
    • 2
  1. 1.Department of BiologyUniversity of WaterlooWaterlooCanada
  2. 2.Department of BiologyWilfrid Laurier UniversityWaterlooCanada