Article

In Vitro Cellular & Developmental Biology - Animal

, Volume 47, Issue 2, pp 104-113

First online:

In vitro growth of microsporidia Anncaliia algerae in cell lines from warm water fish

  • S. Richelle MonaghanAffiliated withDepartment of Biology, University of WaterlooDepartment of Biology, Wilfrid Laurier University Email author 
  • , Rebecca L. RumneyAffiliated withDepartment of Biology, Wilfrid Laurier University
  • , Nguyen T. K. VoAffiliated withDepartment of Biology, Wilfrid Laurier University
  • , Niels C. BolsAffiliated withDepartment of Biology, University of Waterloo
  • , Lucy E. J. LeeAffiliated withDepartment of Biology, Wilfrid Laurier University

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Abstract

Anncaliia algerae is an aquatic microsporidium that most commonly infects mosquitoes but can be grown on the rabbit kidney cell line, RK-13. Spores were purified from RK-13 cultures and added to cell lines from warm water fish and from an insect. The cell lines were GFSK-S1 and GFB3C-W1 from goldfish skin and brain respectively, ZEB2J from zebrafish embryos, FHMT-W1 from fathead minnow testis, and Sf9 from ovaries of a fall armyworm moth. All cultures were maintained at 27°C. Infection was judged to have taken place by the appearance of sporonts and/or spores in cells and occurred in all cell lines. Spores were also isolated from ZEB2J cultures and used to successfully infect new cultures of ZEB2J, RK-13 and Sf9. These results suggest that cells of a wide range of vertebrates support A. algerae growth in vitro and fish cells can produce spores infectious to cells of mammals, fish, and insects

Keywords

Microsporidia Cell lines Goldfish Zebrafish Fathead minnow Anncaliia algerae Brachiola Nosema RK-13 Sf9