Establishment of Asian citrus psyllid (Diaphorina citri) primary cultures
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The Asian citrus psyllid (AsCP), Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is a highly competent vector of the phloem-inhabiting bacterium Candidatus Liberibacter asiaticus associated with the citrus disease huanglongbing (HLB). Commonly referred to as citrus greening disease in the USA, HLB causes reduced fruit yields, quality, and ultimately tree death and is considered the most serious citrus disease. HLB has become a major limiting factor to the production of citrus worldwide. Studies of HLB have been impeded by the fact that C. Liberibacter has not yet been cultured on artificial nutrient media. After being acquired by a psyllid, C. Liberibacter asiaticus is reported to replicate within the psyllid and is retained by the psyllid throughout its life span. We therefore hypothesized that C. Liberibacter asiaticus could be cultured in vitro using psyllid cell cultures as the medium and investigated the establishment of a pure culture for AsCP cells. Several commercially available insect cell culture media along with some media we developed were screened for viability to culture cells from AsCP embryos. Cells from psyllid tissues adhered to the plate and migration was observed within 24 h. Cells were maintained at 20°C. We successfully established primary psyllid cell cultures, referred to as DcHH-1, for D. citri Hert-Hunter-1, with a new media, Hert-Hunter-70.
- Bové J. M. Huanglongbing a destructive, newly-emerging, century-old disease of citrus. J. Plant Pathol. 88: 7–37; 2006.
- Goodwin R. H. Insect cell culture improved media and methods for initiating attached cell lines from the Lepidoptera. In Vitro Cell Dev. Biol.—Plant. 11: 369–378; 1975. doi:10.1007/BF02616373. CrossRef
- Halbert S. E.; Manjunath K. L. Asian citrus psyllids (Sternorrhyncha: Psyllidae) and greening disease of citrus: A literature review and assessment of risk in Florida. Florida Entomol. 87: 7–37; 2004.
- Hung T. H.; Hung S. C.; Chen C. N.; Hsu M. H.; Su H. J. Detection by PCR of Candidatus Liberibacter asiaticus, the bacterium causing citrus huanglongbing in vector psyllids: application to the study of vector–pathogen relationships. Plant Pathol. 53: 96–102; 2004. doi:10.1111/j.1365-3059.2004.00948.x. CrossRef
- Hunter W. B.; Polston J. E. Development of a continuous whitefly cell line (Homoptera: Aleyrodidae: Bemisia tabaci (Genn.) for the study of Begomovirus. J. Invertebr. Pathol. 77: 33–36; 2001. doi:10.1006/jipa.2000.4993. CrossRef
- Kimura I. Establishment of new cell lines from leafhopper vector and inoculation of its cell monolayers with rice dwarf virus. Proc. Jpn. Acad. 60: 198–201; 1984. doi:10.2183/pjab.60.198. CrossRef
- Mitsuhashi J. Primary culture of leafhopper embryonic tissues. J. Tissue Cult. Methods 46122: 151–154; 1976.
- Mitsuhashi J. Invertebrate tissue culture methods (Springer lab manual). Springer, Tokyo-Shoseki, Japan 2002.
- Establishment of Asian citrus psyllid (Diaphorina citri) primary cultures
In Vitro Cellular & Developmental Biology - Animal
Volume 45, Issue 7 , pp 317-320
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