In Vitro Cellular & Developmental Biology - Animal

, Volume 45, Issue 1, pp 44-54

First online:

Culture of endodermal stem/progenitor cells of the mouse tongue

  • Xiaoyan LuoAffiliated withDepartment of Cell Biology, Duke University Medical Center
  • , Tadashi OkuboAffiliated withCenter for Integrative Bioscience, National Institute of Natural Sciences
  • , Scott RandellAffiliated withCystic Fibrosis/Pulmonary Research and Treatment Center, University of North Carolina
  • , Brigid L. M. HoganAffiliated withDepartment of Cell Biology, Duke University Medical Center Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


The tongue represents a very accessible source of tissue-specific epithelial stem cells of endodermal origin. However, little is known about the properties of these cells and the mechanisms regulating their proliferation and differentiation. Foxa2, an endodermal marker, is expressed throughout the tongue epithelium during embryonic development but becomes confined to a minority of basal cells and some taste bud sensory cells in the adult tongue. Using a previously described line of transgenic mice in which enhanced green fluorescent protein (eGFP) is expressed under the control of a human keratin 5 promoter region (Krt5-eGFP), we have isolated a subpopulation of cells in the basal epithelial layer of the mouse tongue with a high efficiency of generating holoclones of undifferentiated cells in culture with a feeder layer. Krt5-GFPhi cells can both self renew and give rise to differentiated stratified keratinized epithelial cells when cultured on an air–liquid interface.


Tongue Endodermal basal stem cell Krt5-eGFP Colony forming efficiency Differentiation