Journal of Huazhong University of Science and Technology [Medical Sciences]

, Volume 33, Issue 1, pp 102–106

Over-expression of heme oxygenase-1 does not protect porcine endothelial cells from human xenoantibodies and complement-mediated lysis

Authors

  • Chi Zhang张 弛
    • Institute of Organ TransplantationHuazhong University of Science and Technology
    • Department of UrologyFujian Provincial Hospital
  • Lu Wang王 璐
    • Institute of Organ TransplantationHuazhong University of Science and Technology
  • Shan Zhong钟 山
    • Institute of Organ TransplantationHuazhong University of Science and Technology
  • Xiao-xiao Wang王筱啸
    • Institute of Organ TransplantationHuazhong University of Science and Technology
  • Ying Xiang向 莹
    • Institute of Organ TransplantationHuazhong University of Science and Technology
  • Shi Chen陈 实
    • Institute of Organ TransplantationHuazhong University of Science and Technology
    • Key Laboratory of Organ Transplantation, Ministry of EducationHuazhong University of Science and Technology
    • Key Laboratory of Organ Transplantation, Ministry of Public Health, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
    • Institute of Organ TransplantationHuazhong University of Science and Technology
    • Key Laboratory of Organ Transplantation, Ministry of EducationHuazhong University of Science and Technology
    • Key Laboratory of Organ Transplantation, Ministry of Public Health, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
Article

DOI: 10.1007/s11596-013-1079-x

Cite this article as:
Zhang, C., Wang, L., Zhong, S. et al. J. Huazhong Univ. Sci. Technol. [Med. Sci.] (2013) 33: 102. doi:10.1007/s11596-013-1079-x

Summary

Accommodated organs can survive in the presence of anti-organ antibodies and complement. Heme oxygenase-1 (HO-1) is essential to ensure accommodation in concordant xenotransplant models. However, whether induction of HO-1 over-expression could protect porcine endothelial cells (PECs) against human xenoantibodies and complement-mediated lysis and induce an in vitro accommodation is still unknown. The SV40-immortalized porcine aorta-derived endothelial cell line (iPEC) was pre-incubated with 20, 50, or 80 μmol/L of cobalt-protoporphyrins IX (CoPPIX) for 24 h, and the HO-1 expression in iPECs was analyzed by using Western blotting. CoPPIX-treated or untreated iPECs were incubated with normal human AB sera, and complement-dependent cytotoxicity (CDC) was measured by both flow cytometry and lactate dehydrogenase (LDH) release assay. In vitro treatment with CoPPIX significantly increased the expression of HO-1 in iPECs in a dose-dependent manner. Over-expression of HO-1 was successfully achieved by incubation of iPECs with either 50 or 80 μmol/L of CoPPIX. However, HO-1 over-expression did not show any protective effects on iPECs against normal human sera-mediated cell lysis. In conclusion, induction of HO-1 over-expression alone is not enough to protect PECs from human xenoantibodies and complement-mediated humoral injury. Additionally, use of other protective strategies is needed to achieve accommodation in pig-to-primate xenotransplantation.

Key words

heme oxygenase-1porcine endothelial cellscomplement-dependent cytotoxicityxenotransplantation

Copyright information

© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2013