Hepatoprotective activity of the ethanol extract of Sarcopyramis Nepalensis
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The present study examined the protective effect of the ethanol extract of Sarcopyramis nepalensis (EESN) on agents-induced hepatotoxicity in mice and the possible mechanism. Acute liver injury was induced by administration of either CCl4 or D-GalN. The animals were divided into 5 groups in terms of different treatment: normal group, CCl4 or D-GalN group, silymarin or bifendate group, low dose EESN group (10 mg/kg) and high dose EESN group (30 mg/kg). Liver function was evaluated by detecting the levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The oxidize stress markers were measured, including malondialdehyde (MDA), glutathione peroxidase (GSH) and superoxide dismutase (SOD). Liver tissues were histopathologically examined by hematoxylin-eosin (H&E) staining. The acute toxicity study revealed that there was no toxicity of EESN at the dose of 5 g/kg in mice. The levels of ALT and AST in serum, and the MDA level in live tissues were significantly increased and the activities of SOD and GSH substantially decreased in mice after CCl4 or D-GalN treatment. These biochemical and oxidize stress markers were profoundly improved after treatment with EESN at different doses, which was similar to the results of silymarin or bifendate treatment. The histophathological examination revealed the significant improvement in the pathological changes of the liver in EESN-treated mice as compared to those in CCl4 or D-GalN group. It was concluded that EESN possesses potential antioxidant and hepatoprotective properties and has therapeutic potential for liver diseases.
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- Hepatoprotective activity of the ethanol extract of Sarcopyramis Nepalensis
Journal of Huazhong University of Science and Technology [Medical Sciences]
Volume 32, Issue 6 , pp 844-848
- Cover Date
- Print ISSN
- Online ISSN
- Huazhong University of Science and Technology
- Additional Links
- Sarcopyramis nepalensis
- hepatoprotective activity
- CCl4, D-GalN
- Industry Sectors
- Author Affiliations
- 1. School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China
- 2. Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Wuhan, 430030, China
- 3. Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China
- 4. College of Chemical Engineering and Pharmacy, Jingchu University of Technology, Jingmen, 448000, China