Journal of Huazhong University of Science and Technology [Medical Sciences]

, Volume 32, Issue 4, pp 511–516

Establishment of reproducible xenotransplantation model of T cell acute lymphoblastic leukemia in NOD/SCID mice

Authors

  • Di Wang王 迪
    • Department of Hematology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
  • Na Wang王 娜
    • Department of Hematology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
  • Yan Zhang张 艳
    • Crown Bioscience Inc. (Beijing)
  • Shuyan Ma马淑燕
    • Crown Bioscience Inc. (Beijing)
  • Zhe Geng耿 哲
    • Department of Hematology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
  • Pengfei Zhou周鹏飞
    • Crown Bioscience Inc. (Beijing)
  • Jianfeng Zhou周剑峰
    • Department of Hematology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
  • Liang Huang黄 亮
    • Department of Hematology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
Article

DOI: 10.1007/s11596-012-0088-5

Cite this article as:
Wang, D., Wang, N., Zhang, Y. et al. J. Huazhong Univ. Sci. Technol. [Med. Sci.] (2012) 32: 511. doi:10.1007/s11596-012-0088-5
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Summary

T cell acute lymphoblastic leukemia (T-ALL) is an aggressive leukemia. However the poor prognosis and low morbidity restrict further analysis of the disease. Therefore there is an increasing demand to develop animal models for identifying novel therapeutic approaches. In this study, we inoculated the anti-mouse CD122 monoclonal antibody conditioned NOD/SCID mice with the leukemia cells from 9 T-ALL patients and 1 cell line via the tail vein. Four of the 9 patients and the cell line were successfully engrafted. Flow cytometry detected high percentage of human CD45+ cells in recipient mice. Immunohistochemistry showed infiltration of human CD45+ cells in different organs. Serial transplantation was also achieved. In vivo drug treatment showed that dexamethasone could extend survival, which was consistent with clinical observation. These results demonstrated that we successfully established 5 xenotransplantation models of T-ALL in anti-mCD122 mAb conditioned NOD/SCID mice, which recapitulated the characteristics of original disease.

Key words

T cell acute lymphoblastic leukemiaxenotransplantationNOD/SCID micein vivo

Copyright information

© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2012