Upregulation of sFlt-1 by trophoblasts induces the barrier dysfunction of glomerular endothelial cells

  • Jun Zhao (赵 君)
  • Haiyi Liu (刘海意)
  • Hui Du (杜 慧)
  • Fuyuan Qiao (乔福元)
  • Yvqi Li (李宇琪)
  • Xinwei Shi (石鑫玮)
  • Xun Gong (龚 洵)
  • Yuanyuan Wu (吴媛媛)
  • Qiong Zhou (周 琼)
  • Jingjing Xu (徐京晶)
Article

DOI: 10.1007/s11596-011-0682-y

Cite this article as:
Zhao, J., Liu, H., Du, H. et al. J. Huazhong Univ. Sci. Technol. [Med. Sci.] (2011) 31: 815. doi:10.1007/s11596-011-0682-y

Summary

This study examined the effect of over-expression of sFlt-1 by trophoblasts on the barrier function of glomerular endothelial cells and the role of VEGF in this process in order to explore the pathogenesis of glomerular disease in preeclampsia. SFlt-1 expression in the human trophoblasts (TEV-1 cells) was enhanced by transfecting sFlt-1 plasmid DNA into TEV-1 cells. The monolayer barrier function of glomerular endothelial cells (ciGEnCs) was determined by measuring the fluorescence intensity of bovine serum albumin (BSA) that crossed the monolayer of glomerular endothelial cells. The results showed that the over-expression of sFlt-1 by TEV-1 cells led to the barrier dysfunction of ciGEnCs, and the exogenous VEGF could alleviate the ciGEnCs dysfunction resulting from the over-expression of sFlt-1 to a certain extent. It was concluded that the dysregulation of sFlt-1 and VEGF in preeclamptic pregnancy may contribute to the barrier dysfunction of glomerular endothelial cells, and VEGF may play an important role in maintaining the barrier function of glomerular endothelial cells, but it may not be the sole factor.

Key words

preeclampsiatrophoblastglomerular endothelial cellmonolayer barrier function

Copyright information

© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Jun Zhao (赵 君)
    • 1
  • Haiyi Liu (刘海意)
    • 1
  • Hui Du (杜 慧)
    • 1
  • Fuyuan Qiao (乔福元)
    • 1
  • Yvqi Li (李宇琪)
    • 1
  • Xinwei Shi (石鑫玮)
    • 1
  • Xun Gong (龚 洵)
    • 1
  • Yuanyuan Wu (吴媛媛)
    • 1
  • Qiong Zhou (周 琼)
    • 1
  • Jingjing Xu (徐京晶)
    • 1
  1. 1.Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina