Article

Journal of Huazhong University of Science and Technology [Medical Sciences]

, Volume 31, Issue 6, pp 779-783

First online:

Isoflurane enhances the expression of cytochrome C by facilitation of NMDA receptor in developing rat hippocampal neurons in vitro

  • Yilin Zhao赵以林Affiliated withDepartment of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
  • , Xiaogao Jin金小高Affiliated withDepartment of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
  • , Jintao Wang王金韬Affiliated withDepartment of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
  • , Lei Tan谭 蕾Affiliated withDepartment of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
  • , Shiyong Li李世勇Affiliated withDepartment of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
  • , Ailin Luo罗爱林Affiliated withDepartment of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Email author 

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Summary

This study examined the effects of clinically relevant concentrations of isoflurane on the amplitude of NMDA receptor current (INMDA) and the expression of cytochrome C in cultured developing rat hippocampal neurons. The hippocampi were dissected from newborn Sprague-Dawley rats. Hippocampal neurons were primarily cultured for 5 days and then treated with different concentrations of isoflurane [(0.25, 0.5, 0.75, 1 minimum alveolar concentration (MAC))]. The peak of INMDA was recorded by means of the whole cell patch clamp technique. The cytochrome C level was detected by Western blotting and quantitative real-time PCR. Our results showed that isoflurane (0.25, 0.5, 0.75 and 1 MAC) potentiated the amplitude of INMDA by (116±8.8)%, (122±11.7)%, (135±14.3)% and (132±14.6)%, respectively, and isoflurane increased the mRNA expression of cytochrome C in a concentration-dependent manner. The cytochrome C mRNA expression reached a maximum after 0.5 MAC isoflurane stimulation for 6 h (P<0.05). It was concluded that isoflurane enhances the expression of cytochrome C in cultured rat hippocampal neurons, which may be mediated by facilitation of NMDA receptor.

Key words

inhalation anesthetic isoflurane hippocampus developing neurons calcium NMDA receptor current