Article

Journal of Huazhong University of Science and Technology [Medical Sciences]

, Volume 31, Issue 1, pp 33-38

First online:

Antitumor activity of erythromycin on human neuroblastoma cell line (SH-SY5Y)

  • Jia Yongsheng贾勇圣Affiliated withBeijing Institute of Pharmacology and Toxicology
  • , Ma Xiaoyun马晓芸Affiliated withBeijing Institute of Pharmacology and Toxicology
  • , Wei Xiaoli魏晓莉Affiliated withBeijing Institute of Pharmacology and Toxicology
  • , Li Xin李 昕Affiliated withBeijing Institute of Pharmacology and Toxicology
  • , Yan Haitao闫海涛Affiliated withBeijing Institute of Pharmacology and Toxicology
  • , Liu Xiaoyan刘晓燕Affiliated withBeijing Institute of Pharmacology and Toxicology
  • , Zheng Jianquan郑建全Affiliated withBeijing Institute of Pharmacology and Toxicology Email author 

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Summary

Antitumor effects of erythromycin and the related mechanism were investigated in the present study. Neuroblastoma cells (SH-SY5Y) were exposed to erythromycin at different concentrations for different durations. Cell proliferation was measured by cell counting, and cell viability was examined by MTT assay. Cell cycle phase distribution and the cytosolic calcium level were detected by flow cytometry. Mitochondrial membrane potential was measured by the JC-1 probe staining and fluorescent microscopy. The expression of an oncogene (c-Myc) and a tumor suppressor [p21 (WAF1/Cip1)] proteins was analyzed by using Western blotting. Erythromycin could inhibit the proliferation of SH-SY5Y cells in a concentration- and time-dependent manner. The cell cycle was arrested at S phase. Mitochondrial membrane potential collapsed and the cytosolic calcium was overloaded in SH-SY5Y cells when treated with erythromycin. The expression of c-Myc protein was down-regulated, while that of p21 (WAF1/Cip1) protein was up-regulated. It was concluded that erythromycin could restrain the proliferation of SH-SY5Y cells. The antitumor mechanism of erythromycin might involve regulating the expression of c-Myc and p21 (WAF1/Cip1) proteins.

Key words

erythromycin c-Myc p21 (WAF1/Cip1) neuroblastoma