Journal of Huazhong University of Science and Technology [Medical Sciences]

, Volume 30, Issue 3, pp 385–390

Aortic cell apoptosis in rat primary aldosteronism model

  • Yongji Yan闫永吉
  • Jinzhi Ouyang欧阳金芝
  • Chao Wang王超
  • Zhun Wu吴 准
  • Xin Ma马 鑫
  • Hongzhao Li李宏召
  • Hua Xu徐 华
  • Zheng Hu胡 争
  • Jun Li李 俊
  • Baojun Wang王保军
  • Taoping Shi史涛坪
  • Daojing Gong龚道静
  • Dong Ni倪 栋
  • Xu Zhang张 旭
Article

DOI: 10.1007/s11596-010-0362-3

Cite this article as:
Yan, Y., Ouyang, J., Wang, C. et al. J. Huazhong Univ. Sci. Technol. [Med. Sci.] (2010) 30: 385. doi:10.1007/s11596-010-0362-3
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Summary

This study aimed to determine whether aldosterone could induce vascular cell apoptosis in vivo. Thirty-two male rats were randomly divided into 4 groups: vehicle (control), aldosterone, aldosterone plus eplerenone or hydralazine. They were then implanted with an osmotic mini-pump that infused either aldosterone or the vehicle. Systolic blood pressure (SBP) was measured weekly by the tail-cuff method. After 8 weeks, plasma aldosterone concentration (PAC) and renin activity (PRA) were determined by radioimmunoassay. Aortic apoptosis was examined by TUNEL assay. The levels of cytochrome c and caspase-3 were determined by Western blotting and the expression of Bax and Bcl-2 was detected by immnuohistochemistry and Western blotting. The results showed that as compared with control group, aldosterone-infused rats exhibited: (1) an increase in SBP; (2) significantly elevated PAC with depressed PRA; (3) elevated aortic vascular cell apoptosis accompanied with higher levels of cytochrome c and activated caspase-3; and (4) significantly up-regulated Bax protein with down-regulated Bcl-2. These effects of aldosterone were significantly inhibited after co-administration with eplerenone but not with hydralazine. It was concluded that aldosterone induced vascular cell apoptosis by its direct effect on the aorta via mineralocorticoid receptors and independently of blood pressure, which may contribute to aldosterone-mediated vascular injury.

Key words

aldosteronismapoptosismineralocorticoid receptoraorta

Copyright information

© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Yongji Yan闫永吉
    • 1
    • 2
  • Jinzhi Ouyang欧阳金芝
    • 3
  • Chao Wang王超
    • 4
  • Zhun Wu吴 准
    • 1
  • Xin Ma马 鑫
    • 2
  • Hongzhao Li李宏召
    • 2
  • Hua Xu徐 华
    • 1
  • Zheng Hu胡 争
    • 1
  • Jun Li李 俊
    • 1
  • Baojun Wang王保军
    • 2
  • Taoping Shi史涛坪
    • 2
  • Daojing Gong龚道静
    • 1
  • Dong Ni倪 栋
    • 1
  • Xu Zhang张 旭
    • 1
    • 2
  1. 1.Department of Urology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Urology, Chinese People’s Liberation Army General HospitalMilitary Postgraduate Medical CollegeBeijingChina
  3. 3.Department of Endocrinology, Chinese People’s Liberation Army General HospitalMilitary Postgraduate Medical CollegeBeijingChina
  4. 4.Department of UrologyJining First People’s HospitalJiningChina