Journal of Huazhong University of Science and Technology [Medical Sciences]

, Volume 30, Issue 3, pp 385–390

Aortic cell apoptosis in rat primary aldosteronism model

Authors

  • Yongji Yan闫永吉
    • Department of Urology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
    • Department of Urology, Chinese People’s Liberation Army General HospitalMilitary Postgraduate Medical College
  • Jinzhi Ouyang欧阳金芝
    • Department of Endocrinology, Chinese People’s Liberation Army General HospitalMilitary Postgraduate Medical College
  • Chao Wang王超
    • Department of UrologyJining First People’s Hospital
  • Zhun Wu吴 准
    • Department of Urology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
  • Xin Ma马 鑫
    • Department of Urology, Chinese People’s Liberation Army General HospitalMilitary Postgraduate Medical College
  • Hongzhao Li李宏召
    • Department of Urology, Chinese People’s Liberation Army General HospitalMilitary Postgraduate Medical College
  • Hua Xu徐 华
    • Department of Urology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
  • Zheng Hu胡 争
    • Department of Urology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
  • Jun Li李 俊
    • Department of Urology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
  • Baojun Wang王保军
    • Department of Urology, Chinese People’s Liberation Army General HospitalMilitary Postgraduate Medical College
  • Taoping Shi史涛坪
    • Department of Urology, Chinese People’s Liberation Army General HospitalMilitary Postgraduate Medical College
  • Daojing Gong龚道静
    • Department of Urology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
  • Dong Ni倪 栋
    • Department of Urology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
    • Department of Urology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
    • Department of Urology, Chinese People’s Liberation Army General HospitalMilitary Postgraduate Medical College
Article

DOI: 10.1007/s11596-010-0362-3

Cite this article as:
Yan, Y., Ouyang, J., Wang, C. et al. J. Huazhong Univ. Sci. Technol. [Med. Sci.] (2010) 30: 385. doi:10.1007/s11596-010-0362-3
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Summary

This study aimed to determine whether aldosterone could induce vascular cell apoptosis in vivo. Thirty-two male rats were randomly divided into 4 groups: vehicle (control), aldosterone, aldosterone plus eplerenone or hydralazine. They were then implanted with an osmotic mini-pump that infused either aldosterone or the vehicle. Systolic blood pressure (SBP) was measured weekly by the tail-cuff method. After 8 weeks, plasma aldosterone concentration (PAC) and renin activity (PRA) were determined by radioimmunoassay. Aortic apoptosis was examined by TUNEL assay. The levels of cytochrome c and caspase-3 were determined by Western blotting and the expression of Bax and Bcl-2 was detected by immnuohistochemistry and Western blotting. The results showed that as compared with control group, aldosterone-infused rats exhibited: (1) an increase in SBP; (2) significantly elevated PAC with depressed PRA; (3) elevated aortic vascular cell apoptosis accompanied with higher levels of cytochrome c and activated caspase-3; and (4) significantly up-regulated Bax protein with down-regulated Bcl-2. These effects of aldosterone were significantly inhibited after co-administration with eplerenone but not with hydralazine. It was concluded that aldosterone induced vascular cell apoptosis by its direct effect on the aorta via mineralocorticoid receptors and independently of blood pressure, which may contribute to aldosterone-mediated vascular injury.

Key words

aldosteronismapoptosismineralocorticoid receptoraorta
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© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2010