Article

Journal of Huazhong University of Science and Technology [Medical Sciences]

, Volume 30, Issue 3, pp 385-390

First online:

Aortic cell apoptosis in rat primary aldosteronism model

  • Yongji Yan闫永吉Affiliated withDepartment of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Urology, Chinese People’s Liberation Army General Hospital, Military Postgraduate Medical College
  • , Jinzhi Ouyang欧阳金芝Affiliated withDepartment of Endocrinology, Chinese People’s Liberation Army General Hospital, Military Postgraduate Medical College
  • , Chao Wang王超Affiliated withDepartment of Urology, Jining First People’s Hospital
  • , Zhun Wu吴 准Affiliated withDepartment of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
  • , Xin Ma马 鑫Affiliated withDepartment of Urology, Chinese People’s Liberation Army General Hospital, Military Postgraduate Medical College
  • , Hongzhao Li李宏召Affiliated withDepartment of Urology, Chinese People’s Liberation Army General Hospital, Military Postgraduate Medical College
  • , Hua Xu徐 华Affiliated withDepartment of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
  • , Zheng Hu胡 争Affiliated withDepartment of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
  • , Jun Li李 俊Affiliated withDepartment of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
    • , Baojun Wang王保军Affiliated withDepartment of Urology, Chinese People’s Liberation Army General Hospital, Military Postgraduate Medical College
    • , Taoping Shi史涛坪Affiliated withDepartment of Urology, Chinese People’s Liberation Army General Hospital, Military Postgraduate Medical College
    • , Daojing Gong龚道静Affiliated withDepartment of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
    • , Dong Ni倪 栋Affiliated withDepartment of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
    • , Xu Zhang张 旭Affiliated withDepartment of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Urology, Chinese People’s Liberation Army General Hospital, Military Postgraduate Medical College Email author 

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Summary

This study aimed to determine whether aldosterone could induce vascular cell apoptosis in vivo. Thirty-two male rats were randomly divided into 4 groups: vehicle (control), aldosterone, aldosterone plus eplerenone or hydralazine. They were then implanted with an osmotic mini-pump that infused either aldosterone or the vehicle. Systolic blood pressure (SBP) was measured weekly by the tail-cuff method. After 8 weeks, plasma aldosterone concentration (PAC) and renin activity (PRA) were determined by radioimmunoassay. Aortic apoptosis was examined by TUNEL assay. The levels of cytochrome c and caspase-3 were determined by Western blotting and the expression of Bax and Bcl-2 was detected by immnuohistochemistry and Western blotting. The results showed that as compared with control group, aldosterone-infused rats exhibited: (1) an increase in SBP; (2) significantly elevated PAC with depressed PRA; (3) elevated aortic vascular cell apoptosis accompanied with higher levels of cytochrome c and activated caspase-3; and (4) significantly up-regulated Bax protein with down-regulated Bcl-2. These effects of aldosterone were significantly inhibited after co-administration with eplerenone but not with hydralazine. It was concluded that aldosterone induced vascular cell apoptosis by its direct effect on the aorta via mineralocorticoid receptors and independently of blood pressure, which may contribute to aldosterone-mediated vascular injury.

Key words

aldosteronism apoptosis mineralocorticoid receptor aorta