A possible mechanism linking hyperglycemia and reduced high-density lipoprotein cholesterol levels in diabetes

  • Feng Gao (高 峰)
  • Tong Yan (严 同)
  • Yan Zhao (赵 艳)
  • Fan Yin (尹 凡)
  • Cuining Hu (胡翠宁)
Article

DOI: 10.1007/s11596-010-0349-0

Cite this article as:
Gao, F., Yan, T., Zhao, Y. et al. J. Huazhong Univ. Sci. Technol. [Med. Sci.] (2010) 30: 318. doi:10.1007/s11596-010-0349-0

Summary

This study investigated the role of glucose in the biogenesis of high-density lipoprotein cholesterol (HDL-C). Mouse primary peritoneal macrophages were harvested and maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing glucose of various concentrations. The cells were divided into 3 groups in terms of different glucose concentrations in the cultures: Control group (5.6 mmol/L glucose), high glucose concentration groups (16.7 mmol/L and 30 mmol/L glucose). ATP-binding cassette transporter A1 (ABCA1) mRNA expression in the macrophages was detected by semi-quantitative RT-PCR 24, 48 and 72 h after glucose treatment. The results showed that ABCA1 mRNA expression in the 16.7 mmol/L glucose group was not significantly different from that in the control group at all testing time points (P>0.05 for each). In the 30 mmol/L glucose group, macrophage ABCA1 mRNA expression was not changed significantly at 24 h (P=0.14), but was substantially decreased by 40.4% at 48 h (P=0.009) and by 48.1% at 72 h (P=0.015) as compared with that in the control group. It was concluded that ABCA1 is of vital importance for HDL-C biogenesis. High glucose may hamper HDL-C biogenesis by decreasing ABCA1 expression, which contributes to low HDL-C level in diabetes.

Key words

reverse cholesterol transportdiabeteshigh-density lipoprotein cholesterolATP-binding cassette transporter A1

Copyright information

© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Feng Gao (高 峰)
    • 1
  • Tong Yan (严 同)
    • 1
  • Yan Zhao (赵 艳)
    • 1
  • Fan Yin (尹 凡)
    • 1
  • Cuining Hu (胡翠宁)
    • 1
  1. 1.Department of Endocrinology, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina