Article

Journal of Huazhong University of Science and Technology [Medical Sciences]

, Volume 30, Issue 1, pp 98-102

First online:

In vitro anti-hepatitis B virus effect of Hypericum perforatum L.

  • Ran Pang庞 然Affiliated withDepartment of Hepatology and Infectious Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
  • , Junyan Tao陶君彦Affiliated withCollege of Pharmacy, Hubei College of Traditional Chinese Medicine
  • , Shuling Zhang张淑玲Affiliated withDepartment of Hepatology and Infectious Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology Email author 
  • , Jiang Zhu朱 江Affiliated withFood Industry Research Institute of Jiangxi Province
  • , Xin Yue乐 鑫Affiliated withState Key Laboratory of Virology, College of Life Sciences, Wuhan University
  • , Lei Zhao赵 雷Affiliated withDepartment of Hepatology and Infectious Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
  • , Pian Ye叶 翩Affiliated withDepartment of Hepatology and Infectious Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
  • , Ying Zhu朱 应Affiliated withState Key Laboratory of Virology, College of Life Sciences, Wuhan University

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Summary

The anti-hepatitis B virus (HBV) effects and its mechanisms of the ethanol extracts of Hypericum perforatum L. (EHP) in vitro were explored. HepG2 2.2.15 cells, a stable HBV-producing cell line, were cultured as the model system to observe the anti-HBV effect. The viral antigens of cellular secretion, HBsAg and HBeAg, were determined by enzyme linked immunosorbent assay (ELISA). The quantity of HBV-DNA released in the supernatant was assayed by real-time PCR. In order to understand the mechanisms of the suppression of HBV replication, all HBV promoters (Cp, Xp, S1p, S2p and Fp) with luciferase reporter gene were transfected into HepG2 cells respectively. Then the activities of viral promoters were examined by luciferase reporter assay. It was found EHP effectively suppressed the secretion of HBsAg and HBeAg from HepG2 2.2.15 cells in a dose-dependent manner, as well as the extracellular HBV DNA. And EHP could selectively inhibit the activity of HBV promoter Fp. Our data suggest that EHP exerts anti-HBV effects via inhibition of HBV transcription, which helps to elucidate the mechanism underlying the potential therapeutic value of EHP.

Key words

hepatitis B virus Hypericum perforatum L. HBsAg HBeAg HBV DNA HBV promoter