Journal of Huazhong University of Science and Technology [Medical Sciences]

, Volume 29, Issue 5, pp 585–591

Characterization of acute renal allograft rejection by human serum proteomic analysis

Authors

  • Ying Gao高 英
    • Institute of Organ Transplantation, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology, Key Laboratory of Organ Transplantation, Ministry of Education, Ministry of Health
  • Ke Wu吴 轲
    • Institute of Organ Transplantation, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology, Key Laboratory of Organ Transplantation, Ministry of Education, Ministry of Health
  • Yi Xu徐 逸
    • Institute of Organ Transplantation, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology, Key Laboratory of Organ Transplantation, Ministry of Education, Ministry of Health
  • Hongmin Zhou周鸿敏
    • Institute of Organ Transplantation, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology, Key Laboratory of Organ Transplantation, Ministry of Education, Ministry of Health
  • Wentao He何文涛
    • Institute of Organ Transplantation, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology, Key Laboratory of Organ Transplantation, Ministry of Education, Ministry of Health
  • Weina Zhang张维娜
    • Institute of Organ Transplantation, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology, Key Laboratory of Organ Transplantation, Ministry of Education, Ministry of Health
  • Lanjun Cai蔡兰军
    • Institute of Organ Transplantation, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology, Key Laboratory of Organ Transplantation, Ministry of Education, Ministry of Health
  • Xingguang Lin林星光
    • Institute of Organ Transplantation, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology, Key Laboratory of Organ Transplantation, Ministry of Education, Ministry of Health
  • Zemin Fang方泽民
    • Institute of Organ Transplantation, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology, Key Laboratory of Organ Transplantation, Ministry of Education, Ministry of Health
  • Zhenlong Luo雒真龙
    • Institute of Organ Transplantation, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology, Key Laboratory of Organ Transplantation, Ministry of Education, Ministry of Health
  • Hui Guo郭 晖
    • Institute of Organ Transplantation, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology, Key Laboratory of Organ Transplantation, Ministry of Education, Ministry of Health
  • Zhonghua Chen陈忠华
    • Institute of Organ Transplantation, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology, Key Laboratory of Organ Transplantation, Ministry of Education, Ministry of Health
Article

DOI: 10.1007/s11596-009-0511-8

Cite this article as:
Gao, Y., Wu, K., Xu, Y. et al. J. Huazhong Univ. Sci. Technol. [Med. Sci.] (2009) 29: 585. doi:10.1007/s11596-009-0511-8

Summary

To identify acute renal allograft rejection biomarkers in human serum, two-dimensional differential in-gel electrophoresis (2-D DIGE) and reversed phase high-performance liquid chromatography (RP-HPLC) followed by electrospray ionization mass spectrometry (ESI-MS) were used. Serum samples from renal allograft patients and normal volunteers were divided into three groups: acute rejection (AR), stable renal function (SRF) and normal volunteer (N). Serum samples were firstly processed using Multiple Affinity Removal Column to selectively remove the highest abundance proteins. Differentially expressed proteins were analyzed using 2-D DIGE. These differential protein spots were excised, digested by trypsin, and identified by RP-HPLC-ESI/MS. Twenty-two differentially expressed proteins were identified in serum from AR group. These proteins included complement C9 precursor, apolipoprotein A-IV precursor, vitamin D-binding protein precursor, beta-2-glycoprotein 1 precursor, etc. Vitamin D-binding protein, one of these proteins, was confirmed by ELISA in the independent set of serum samples. In conclusion, the differentially expressed proteins as serum biomarker candidates may provide the basis of acute rejection noninvasive diagnosis. Confirmed vitamin D-binding protein may be one of serum biomarkers of acute rejection. Furthermore, it may provide great insights into understanding the mechanisms and potential treatment strategy of acute rejection.

Key words

acute rejectiontwo-dimensional differential in-gel electrophoresisreversed phase high-performance liquid chromatographyelectrospray ionization mass spectrometryELISAserum

Copyright information

© Huazhong University of Science and Technology and Springer Berlin Heidelberg 2009