Journal of Huazhong University of Science and Technology [Medical Sciences]

, Volume 29, Issue 2, pp 177–181

ANG II-AT1 receptor pathway is involved in the anti-fibrotic effect of β-elemene

Authors

  • Rui Zhu朱锐
    • Department of Traditional Chinese MedicineHuazhong University of Science and Technology
  • Ling Yang杨玲
    • Huazhong University of Science and Technology
  • Lin Shen沈霖
    • Department of Traditional Chinese MedicineHuazhong University of Science and Technology
  • Jin Ye叶进
    • Huazhong University of Science and Technology
  • Jianguo Liu刘建国
    • Department of Traditional Chinese MedicineHuazhong University of Science and Technology
  • Shenjun Hu胡胜军
    • Department of Traditional Chinese MedicineHuazhong University of Science and Technology
Article

DOI: 10.1007/s11596-009-0208-z

Cite this article as:
Zhu, R., Yang, L., Shen, L. et al. J. Huazhong Univ. Sci. Technol. [Med. Sci.] (2009) 29: 177. doi:10.1007/s11596-009-0208-z

Abstract

To investigate the effects of β-elemene on the ANG II-AT1 receptor pathway in rats with liver fibrosis, a model of hepatic fibrosis was induced by hypodermical injection of carbon tetrachloride (CCl4) into Wistar male rats. β-elemene was intraperitonealy administered into the rats for 8 weeks (0.1 mL/100 g body weight per day). Masson staining was used to observe the liver fibrosis of rats and liver functions were measured by enzymatic kinetic analysis. The content of hydroxyproline in liver tissues was detected by specimen alkaline hydrolysis. The level of plasma ANG in blood II plasma was detected by radioimmunoassay. The expression of AT1R in rat liver were measured using reverse transcriptional-polymerase chain reaction and immunohistochemistry respectively. The results showed that β-elemene could reduce the collagen disposition in liver and inhibit the progression of liver fibrosis. In addition, the levels of plasma ANG II and the expression of hepatic AT1R in rats with liver fibrosis were also suppressed by β-elemene. It is concluded that the ANG II-AT1 receptor pathway plays an important role in the development of hepatic fibrosis and β-elemene could down-regulate the levels of plasma ANG II and the expression of hepatic AT1R in rats with liver fibrosis.

Key words

liver fibrosisβ-elemeneangiotensin IIAT1 receptors

Copyright information

© Huazhong University of Science and Technology and Springer-Verlag GmbH 2009