Journal of Huazhong University of Science and Technology [Medical Sciences]

, Volume 27, Issue 6, pp 625–629

Construction and expression of DNA vaccine pIRES-Sj97-Sj14-Sj26 and its immunogenicity in mice

  • Liu Shuojie 刘朔婕
  • Cheng Jizhong 程继忠
  • Tang Chengwu 唐成武
  • Ma Yanbin 马彦彬
  • Wang Shuyu 王淑玉
  • Guo Ping 郭 萍
  • Duan Qiuhong 段秋红
  • Gao Hong 高 红
  • Dai Wuxing 戴五星
Article

DOI: 10.1007/s11596-007-0601-4

Cite this article as:
Liu, S., Cheng, J., Tang, C. et al. J. Huazhong Univ. Sci. Technol. [Med. Sci.] (2007) 27: 625. doi:10.1007/s11596-007-0601-4

Summary

To find a new preventive strategy for the infection of Schistosoma japonica, plasmid pIRES-Sj97-Sj14-Sj26 that contains fatty binding protein (Sj14), GST (Sj26) and paramyocin (Sj97) that are expressed on the membrane, was constructed. RT-PCR was used to detect the expression of Sj14 mRNA, Sj26 mRNA and Sj97 mRNA in the Hela cells, the indirect immunofluorescent test was employed for the detection of the expression of trans-membrane Sj26 after the plasmid was transfected into Hela cells. Fifty BALB/c mice were randomly divided into 5 groups and pIRES-Sj97-Sj14-Sj26 plasmid DNA, pIRES-Sj14-Sj26 plasmid DNA, pIRES-Sj26 plasmid DNA, pIRES blank vector and normal saline were respectively injected into the quadriceps muscles of thigh. Eight weeks after the immunization the mice were killed and significantly higher level of IgG was detected in the pIRES-Sj97-Sj14-Sj26 group as compared with the pIRES blank vector, normal saline and pIRES-Sj26 groups (P<0.01) and the pIRES-Sj14-Sj26(P<0.05). Single splenocyte suspension was prepared to detected the level of IFN-γ by ELISA and the lymphocyte stimulating index (SI) by MTT. SI was significantly higher of in the pIRES-Sj97-Sj14-Sj26 group than in other groups (P<0.01), while the IFN-γ level was significantly higher the pIRES-Sj97-Sj14-Sj26 group than in pIRES blank vector and normal saline groups (P<0.01), but no significant differences were found when compared with pIRES-Sj14-Sj26 and pIRES-Sj26 groups. Flow cytometery showed that the percent-ages of CD4+ and CD8+ T cells were much higher in the pIRES-Sj97-Sj14-Sj26 group (P< 0.01, P<0.05). It was concluded that pIRES-Sj97-Sj14-Sj26 vaccine may induce stronger immune response in BALB/c mice.

Key words

schistosoma japonicumSj14Sj26Sj97DNA vaccine immunization

Copyright information

© Huazhong University of Science and Technology 2008

Authors and Affiliations

  • Liu Shuojie 刘朔婕
    • 1
  • Cheng Jizhong 程继忠
    • 1
  • Tang Chengwu 唐成武
    • 1
  • Ma Yanbin 马彦彬
    • 1
  • Wang Shuyu 王淑玉
    • 1
  • Guo Ping 郭 萍
    • 1
  • Duan Qiuhong 段秋红
    • 1
  • Gao Hong 高 红
    • 1
  • Dai Wuxing 戴五星
    • 1
  1. 1.Department of Biochemistry and Molecular Biology, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina