The effect of nitrous oxide and isoflurane on the total RNA yield from the cochlea of the rats

  • Li Yuantao  (李元涛)
  • Ke Changbin  (柯昌斌)
  • Yang Jingli  (杨京利)
  • Xiong Liangzhi  (熊良志)
  • Yao Shanglong  (姚尚龙)
Article

DOI: 10.1007/s11596-007-0536-9

Cite this article as:
Li, Y., Ke, C., Yang, J. et al. J. Huazhong Univ. Sci. Technol. [Med. Sci.] (2007) 27: 614. doi:10.1007/s11596-007-0536-9

Summary

The possible mechanism of inhalation anesthetics on the internal auditory impairment of the rat was investigated by determining the effect of nitrous oxide (N2O) and isoflurane on the total RNA yield from the cochlea of the rats. Thirty healthy Wistar rats were randomly divided into 3 groups: group C (control group, n=10) with a 3-h unremitting inhalation of 50% O2, group N (experiment group, n=10) with a continuous inhalation of 50% N2O+50% O2 for 3 h, and group I (experiment group, n=10) with a 3-h sustained inhalation of 2.5% isoflurane. The TRIzol in combination with RNeasy was used to respectively extract the total RNA from cochlea of rats in the 3 groups. Spectrophotometry was used to detect total RNA yield and electrophoresis to detect the quality. The total RNA extracted from the cochlea of the rats in the groups C and N was 7.69 and 6.51 μg, respectively. There was a 15% decrease in the N group as compared with group C. The total RNA from the rats in the group I was 7.32 μg, and there was hardly any change in the group as compared with the group C. The value of A260/A280 in groups C, N and I was 2.07, 2.04 and 2.04, respectively, showing a very high RNA purity. The result of gel electrophoresis suggested that there was no degradation in the total RNA. It was suggested that the interference of N2O on the cochlear RNA yield might be one of the reasons which cause an injury of the ear. The isoflurane shows no harm on the hearing.

Key words

nitrous oxide isoflurane cochlea total RNA yield 

Copyright information

© Huazhong University of Science and Technology 2007

Authors and Affiliations

  • Li Yuantao  (李元涛)
    • 1
    • 2
  • Ke Changbin  (柯昌斌)
    • 2
  • Yang Jingli  (杨京利)
    • 2
  • Xiong Liangzhi  (熊良志)
    • 2
  • Yao Shanglong  (姚尚龙)
    • 1
  1. 1.Department of Anesthesiology, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Department of AnesthesiologyAffiliated Shiyan Taihe Hospital of Yunyang Medical CollegeShiyanChina