Journal of Huazhong University of Science and Technology

, Volume 27, Issue 2, pp 135–137

Effect of Radix Isatidis on the expression of moesin mRNA induced by LPS in the tissues of mice

Authors

  • Li Jing 李敬
    • Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
  • Liu Yunhai 刘云海
    • Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
  • Fang Jianguo 方建国
    • Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
  • Chen Xin 陈新
    • Department of Biotechnology and Pharmacy EngineeringWuhan Polytechnic University
  • Xie Wei 谢委
    • Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
Article

DOI: 10.1007/s11596-007-0206-y

Cite this article as:
Li, J., Liu, Y., Fang, J. et al. J. Huazhong Univ. Sc. Technol. (2007) 27: 135. doi:10.1007/s11596-007-0206-y

Summary

To investigate the effect of the anti-endotoxic part of Radix Isatidis on the expression of moesin mRNA in murine tissues induced by lipopolysaccharide (LPS), the sample solution of F022 part from Radix Isatidis was intraperitoneally administered to experimental mice, and the lipopoly-saccharide (LPS) were injected into the tail vein, and then the tissues of liver, kidney and spleen were colleted and cut into slices. The mRNA was detected by moesin mRNA hybridization in situ. The staining results were observed under microscope. It was found that moesin mRNA expression was increased in the tissues of liver, kidndy and spleen in mice treated with LPS, while in the mice pre-treated with F022 part from Radix Isatidis, the LPS-induced moesin mRNA expressions in these tissues were inhibited in a dose-dependant manner. Our study showed that F022 part from Radix Isatidis can inhibit the LPS-induced expression of moesin mRNA in the tissues of liver, kidney and spleen in mice.

Key words

Radix Isatidislipopolysaccharide (LPS)membrane-organizing extension spike protein mRNA (moesin mRNA)

Copyright information

© Huazhong University of Science and Technology 2007