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Three novel species of Stemphylium from Sinkiang, China: their morphological and molecular characterization

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Abstract

Three new species of Stemphylium were isolated from diseased leaves of Luffa cylindrica, Lycium chinense and Cucumis melo growing in the Sinkiang province of Northwest China. Stemphylium luffae, S. lycii and S. cucumis are described by morphological and molecular phylogenetic analyses. The principal morphological characteristics of these three species are congruent with those of Stemphylium. They are distinguished from morphologically similar Stemphylium species based on characteristics of their conidia and conidiophores. In order to establish the molecular phylogenetic status of these species, DNA loci including the internal transcribed spacer (ITS) nuclear rDNA region and the glyceraldehyde-3-phosphate dehydrogenase (gpd) genes were amplified and sequenced. Phylogenetic analyses using Maximum-Parsimony (MP) and Neighbor-Joining (NJ) were performed on the combined DNA sequences of ITS and gpd gene regions. Both morphological observations and molecular analyses support S. luffae, S. lycii and S. cucumis as three new taxa.

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Acknowledgment

This work was supported by the National Natural Science Foundation of China (no. 30570006).

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Authors and Affiliations

  1. Department of Plant Pathology, Shandong Agricultural University, Taian, 271018, China

    Yun-Fei Pei, Yong Wang, Yun Geng & Xiu-Guo Zhang

  2. Systematic Mycology and Microbiology Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, 20705-2350, USA

    Nichole R. O’Neill

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  1. Yun-Fei Pei
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  2. Yong Wang
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  3. Yun Geng
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  4. Nichole R. O’Neill
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  5. Xiu-Guo Zhang
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Correspondence to Xiu-Guo Zhang.

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Pei, YF., Wang, Y., Geng, Y. et al. Three novel species of Stemphylium from Sinkiang, China: their morphological and molecular characterization. Mycol Progress 10, 163–173 (2011). https://doi.org/10.1007/s11557-010-0686-1

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  • DOI: https://doi.org/10.1007/s11557-010-0686-1

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