Skip to main content
SpringerLink
Log in

Oryzalin Treatment of Potato Diploids Yields Tetraploid and Chimeric Plants from which Euploids could be Derived by Callus Induction

  • Published:
Potato Research Aims and scope Submit manuscript

Abstract

Shoot apices from six diploid potato genotypes for polyploidisation were treated with oryzalin and colchicine solutions at different concentrations and incubation times. In addition to chimeric plants, diploids, one octoploid and completely ploidy doubled plants (4x) were regenerated in these experiments. Tetraploid plants were obtained for three of the six genotypes treated with oryzalin, and no tetraploid plants were obtained after colchicine treatments of any of the genotypes. The best result of 43% tetraploid plants was achieved using 28.8 μM oryzalin and 24 h of incubation with the L37 genotype. Moreover, we determined that doubled plants should not be selected on the basis of a single measurement and require additional ploidy checks in successive clonal generations. Some L37 plants that were initially identified as tetraploids by flow cytometry produced diploid or mixoploid plants in the next clonal generation. Morphological characters of doubled L37 plants were compared with the original genotype. The PVY resistance present in a parental clone was maintained in all regenerants and significant differences among ploidy variants were found for some quantitative characters. Molecular AFLP analyses did not show any differences among genotypes. Since polyploidizing substances may produce a large proportion of chimeric plants, we applied a method to increase the number of tetraploid plants. Callus culture of chimeric leaf explants was performed on regeneration medium and tetraploid shoots were obtained in four of the six genotypes tested. The best result of 40% tetraploid plants was achieved with explants from the L37 genotype. This method was successful in producing additional tetraploid genotypes from mixoploid plants obtained after oryzalin treatments.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barandalla L, Sanchez I, Ritter E, Ruiz de Galarreta JI (2003) Conservation of potato (Solanum tuberosum L.) cultivars by cryopreservation. Span J Agric Res 1:9–13

    Google Scholar 

  • Bartels PG, Hilton JL (1973) Comparison of trifuralin, oryzalin, pronamide, propham, and colchicine treatments on microtubules. Pestic Biochem Physiol 3:462–472

    Article  CAS  Google Scholar 

  • Brown SC, Bergounioux C, Tallet S, Marie D (1991) Flow cytometry of nuclei for ploidy and cell cycle analysis. In: Negrutiu I, Gharti-Chhetri G (eds) A laboratory guide for cellular and molecular plant biology. Basel, Bikhaüser, pp326–345

    Google Scholar 

  • Cardi T, Iannamico V, Dambrosio F, Filippone E, Lurquin PF (1993) In vitro shoot regeneration and cytological characterization of shoots from leaf explants of 3 accessions of Solanum commersonii. Plant Cell, Tissue Organ Cult 34:107–114

    Article  Google Scholar 

  • Chauvin JE, Souchet C, Dantec JP, Ellisséche D (2003) Chromosome doubling of 2x Solanum species by oryzalin: method development and comparison with spontaneous chromosome doubling in vitro. Plant Cell, Tissue Organ Cult 73:65–73

    Article  CAS  Google Scholar 

  • Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol 34:475–483

    PubMed  CAS  Google Scholar 

  • Crofts N, Maskill W, Gust ID (1988) Evaluation of enzyme-linked immunosorbent assay: a method of data analysis. J Virol Methods 15:233–247

    Google Scholar 

  • De Maine MJ, Fantes JA (1983) The results of colchicine treatment of dihaploids and their implications regarding the efficiency of chromosome doubling and potato histogeny. Potato Res 26:289–294

    Article  Google Scholar 

  • De Maine MJ, Stewart HE (1988) An assessment of the fertilizer response and other characters of three dihaploid potato clones and the characteristics of their tetraploid progenies. Euphytica 39:25–31

    Article  Google Scholar 

  • De Maine MJ, Simpson G (1999) Somatic chromosome number doubling of selected potato genotypes using callus culture or the colchicine treatment of shoot nodes in vitro. Ann Appl Biol 134:125–130

    Google Scholar 

  • Dolezël J (1991) Flow cytometric analysis of nuclear DNA content in higher plants. Phytochem Anal 2:143–154

    Article  Google Scholar 

  • Dolezël J, Sgorbati S, Lucretti S (1992) Comparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plants. Physiol Plant 85:625–631

    Article  Google Scholar 

  • Fleming MLMH, De Maine MJ, Powell W (1992) Ploidy doubling by callus culture of potato dihaploid leaf explants and the variation in regenerated plants. Ann Appl Biol 121:183–188

    Article  Google Scholar 

  • Ghislain M, Zhang D, Fajardo D, Huamán Z, Hijmans RJ (1999) Marker-assisted sampling of the cultivated Andean potato Solanum phureja collection using RAPD markers. Genet Resour Crop Evol 46:547–555

    Article  Google Scholar 

  • Golmirzaie AM, Mendoza HM (1988) Breeding strategies for true potato seed production. In CIP circular, vol 16, no 4. International Potato Center, Lima, Perú

  • Görg R, Schachtschabel U, Ritter E, Salamini F, Gebhardt C (1992) Discrimination among 136 tetraploid potato varieties by fingerprints using highly polymorphic DNA markers. Crop Sci 32:815–819

    Article  Google Scholar 

  • Hulme JS, Higgins ES, Shields R (1992) An efficient genotype-independent method for regeneration of potato plants from leaf tissue. Plant Cell, Tissue Organ Cult 31:161–167

    Article  CAS  Google Scholar 

  • Karp A, Jones MGK, Foulger D, Fish N, Bright SWJ (1989) Variability in potato tissue culture. Am Potato J 66:669–684

    Google Scholar 

  • Kim JH, Joung H, Kim HY, Lim YP (1998) Estimation of genetic variation and relationship in potato (Solanum tuberosum L.) cultivars using AFLP markers. Am J Potato Res 75:107–112

    Article  CAS  Google Scholar 

  • LI-COR 4200 (1997) Automatic DNA sequencer, MWG Biotech, Ebersberg, Germany

  • McGregor CE, Lambert CA, Greyling MM, Louw JH, Warnich L (2000) A comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR) in tetraploid potato (Solanum tuberosum L.) germplasm. Euphytica 113:135–144

    Article  CAS  Google Scholar 

  • McGregor CE, Van Treuren R, Hoekstra R, Van Hintum TJL (2002) Analysis of the wild potato germplasm of the series Acaulia with AFLPs: implications for ex situ conservation. Theor Appl Genet 104:146–156

    Article  PubMed  CAS  Google Scholar 

  • Miller G (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:26

    Google Scholar 

  • Mozafari J, Wolyn DJ, Alikhan ST (1997) Chromosome doubling via tuber disc culture in dihaploid potato as determined by confocal microscopy. Plant Cell Rep 16:329–333

    CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Ramulu KS, Verhoeven HA, Dijkhuis P (1991) Mitotic blocking, micronucleation, and chromosome doubling by oryzalin, amiprophos-methyl, and colchicine in potato. Protoplasma 160:65–71

    Article  CAS  Google Scholar 

  • Ritter E, Lucca F, Sánchez I, Ruiz de Galarreta JI, Aragonés A, Castañón S, Bryan G, Waugh R, Lefebvre V, Rousselle-Bourgoise F, Gebhardt C, van Eck H, van Os H, Taco J, Baker J (2005) Genomic resources in potato and possibilities for exploitation. In: Haverkort AJ, Struik PC (eds) Potato in progress, science meets practice. Wageningen, The Netherlands, pp55–65

    Google Scholar 

  • Ross RW, Dionne LA, Hougas RW (1967) Doubling the chromosome number of selected Solanum genotypes. Eur Potato J 10:37–52

    Article  Google Scholar 

  • Rousselle P, Rousselle-Bourgeois F (1989) Application de la culture in vitro au doublement chromosomique chez la pomme de terre. Cinquantenaire de la culture in vitro chez les végétaux. In: Doré (ed) Versalles, 24–25 October 1989, C. Colloques del INRA, vol 51 321 pp

  • SAS (2000) SAS/STAT Users Guide, version 6, vol 2, 4th edn, SAS Institute Inc., Cary, NC

    Google Scholar 

  • Simmonds NW (1977) Relation between specific gravity, dry matter content and starch content of potatoes. Potato Res 20:137–140

    Article  Google Scholar 

  • Uijtewaal BA, Jacobsen E, Hermsen JGTh (1987) Morphology and vigour of monohaploid potato clones, their corresponding homozygous diploids and tetraploids and their heterozygous diploid parent. Euphytica 36:745–753

    Article  Google Scholar 

  • Van Breukelen EWM, Ramanna MS, Hermsen JGT (1977) Parthenogenetic monohaploids 2n = X = 12 from Solanum tuberosum L. and Solanum verrucosum Schlechtd and production of homozygous potato diploids. Euphytica 26:263–271

    Article  Google Scholar 

  • Veilleux RE (1996) Haploids in important crop plants – potato. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, vol 3: important selected plants. Kluwer, Dordrecht, pp 37–49

    Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

  • Wheeler VA, Evans NE, Foulger D, Webb KJ, Karp A, Franklin J, Bright SWJ (1985) Shoot formation from explant cultures of fourteen potato cultivars and studies of the cytology and morphology of regenerated plants. Ann Bot 55:309–320

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. NEIKER, Instituto Vasco de Investigación y Desarrollo Agrario, P.O. Box 46, E01080, Vitoria, Spain

    Leire Barandalla, Enrique Ritter & Jose Ignacio Ruiz De Galarreta

Authors
  1. Leire Barandalla
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Enrique Ritter
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jose Ignacio Ruiz De Galarreta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jose Ignacio Ruiz De Galarreta.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Barandalla, L., Ritter, E. & Ruiz De Galarreta, J.I. Oryzalin Treatment of Potato Diploids Yields Tetraploid and Chimeric Plants from which Euploids could be Derived by Callus Induction. Potato Res. 49, 143–154 (2006). https://doi.org/10.1007/s11540-006-9014-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11540-006-9014-1

Keywords

Search

Navigation