Medical & Biological Engineering & Computing

, Volume 48, Issue 10, pp 965–976

Microfabricated substrates as a tool to study cell mechanotransduction

  • Jimmy le Digabel
  • Marion Ghibaudo
  • Léa Trichet
  • Alain Richert
  • Benoit Ladoux
Special Issue - Review

DOI: 10.1007/s11517-010-0619-9

Cite this article as:
le Digabel, J., Ghibaudo, M., Trichet, L. et al. Med Biol Eng Comput (2010) 48: 965. doi:10.1007/s11517-010-0619-9

Abstract

Mechanical cell–substrate interactions affect many cellular functions such as spreading, migration, and even differentiation. These interactions can be studied by incorporating micro- and nanotechnology-related tools. The design of substrates based on these technologies offers new possibilities to probe the cellular responses to changes in their physical environment. The investigations of the mechanical interactions of cells and their surrounding matrix can be carried out in well-defined and near physiological conditions. In particular, this includes the transmission of forces as well as rigidity and topography sensing mechanisms. Here, we review techniques and tools based on nano- and micro-fabrication that have been developed to analyze the influence of the mechanical properties of the substrate on cell functions. We also discuss how microfabrication methods have improved our knowledge on cell adhesion and migration and how they could solve remaining problems in the field of mechanobiology.

Keywords

MicrofabricationCell mechanicsSubstrate stiffnessCell contractilityIntegrinsTopographyMechanotransduction

Copyright information

© International Federation for Medical and Biological Engineering 2010

Authors and Affiliations

  • Jimmy le Digabel
    • 1
  • Marion Ghibaudo
    • 1
  • Léa Trichet
    • 1
  • Alain Richert
    • 1
  • Benoit Ladoux
    • 1
  1. 1.Laboratoire Matière et Systèmes Complexes (MSC)CNRS UMR 7057 & Université Paris DiderotParisFrance