Medical & Biological Engineering & Computing

, 47:41

Neuregulin-1 enhances differentiation of cardiomyocytes from embryonic stem cells

Authors

  • Zhi Wang
    • Department of Cardiology, The First People’s HospitalShanghai Jiaotong University School of Medicine
  • Guotong Xu
    • Key Laboratory of Stem Cell Biology, Institute of Health Sciences and Institute of Biochemistry and Cell BiologyShanghai Institute for Biological Science, Chinese Academy of Science
  • Yalan Wu
    • Key Laboratory of Stem Cell Biology, Institute of Health Sciences and Institute of Biochemistry and Cell BiologyShanghai Institute for Biological Science, Chinese Academy of Science
  • Yuan Guan
    • Key Laboratory of Stem Cell Biology, Institute of Health Sciences and Institute of Biochemistry and Cell BiologyShanghai Institute for Biological Science, Chinese Academy of Science
  • Lu Cui
    • Key Laboratory of Stem Cell Biology, Institute of Health Sciences and Institute of Biochemistry and Cell BiologyShanghai Institute for Biological Science, Chinese Academy of Science
  • Xia Lei
    • Key Laboratory of Stem Cell Biology, Institute of Health Sciences and Institute of Biochemistry and Cell BiologyShanghai Institute for Biological Science, Chinese Academy of Science
  • Jingfa Zhang
    • Key Laboratory of Stem Cell Biology, Institute of Health Sciences and Institute of Biochemistry and Cell BiologyShanghai Institute for Biological Science, Chinese Academy of Science
  • Lisha Mou
    • Key Laboratory of Stem Cell Biology, Institute of Health Sciences and Institute of Biochemistry and Cell BiologyShanghai Institute for Biological Science, Chinese Academy of Science
  • Baogui Sun
    • Department of Cardiology, The First People’s HospitalShanghai Jiaotong University School of Medicine
    • Department of Cardiology, The First People’s HospitalShanghai Jiaotong University School of Medicine
Original Article

DOI: 10.1007/s11517-008-0383-2

Cite this article as:
Wang, Z., Xu, G., Wu, Y. et al. Med Biol Eng Comput (2009) 47: 41. doi:10.1007/s11517-008-0383-2

Abstract

Neuregulin-1 (NRG-1) is a multifunctional regulator that acts through receptor tyrosine kinases of the epidermal growth factor (EGF/ErbB) receptor family in diverse tissue. ErbB receptors are expressed in developing embryoid bodies (EBs), and the importance of the NRG-1/ErbB signaling axis in heart development has been investigated, but the underlying molecular mechanism is poorly studied. NRG-1 treatment at 100 ng/ml significantly increased the number of beating EBs of differentiated murine embryonic stem cells (ESCs). Furthermore, NRG-1 up-regulated the expression of the cardiac-restricted transcription factors Nkx2.5 and GATA-4 and factors involved in differentiated cardiac cells (α-MHC, β-MHC and α-actinin); NRG-1-induced increase of Nkx2.5 transcription was inhibited by treatment with the PI3 K inhibitor or ErbB receptor inhibitor. Western blot analysis confirmed that the expression of phospho-Akt in the beating foci was increased in the presence of NRG-1. Our results suggest that NRG-1 promotes cardiomyocyte differentiation of ESCs and the ErbB/PI3 K/Akt signaling pathway is one of the underlying molecular mechanisms.

Keywords

Neuregulin-1 Embryonic stem cells Cardiomyocytes Phosphatidylinositol 3-kinase

Copyright information

© International Federation for Medical and Biological Engineering 2008