Journal of Neuroimmune Pharmacology

, Volume 6, Issue 3, pp 362–370

M. tuberculosis H37Rv Infection of Chinese Rhesus Macaques

  • Jing Zhang
  • Yan-Qing Ye
  • Yong Wang
  • Ping-Zheng Mo
  • Qiao-Yang Xian
  • Yan Rao
  • Rong Bao
  • Ming Dai
  • Jun-Yan Liu
  • Ming Guo
  • Xin Wang
  • Zhi-Xiang Huang
  • Li-Hua Sun
  • Zhi-Jiao Tang
  • Wen-Zhe Ho
ORIGINAL ARTICLE

DOI: 10.1007/s11481-010-9245-4

Cite this article as:
Zhang, J., Ye, Y., Wang, Y. et al. J Neuroimmune Pharmacol (2011) 6: 362. doi:10.1007/s11481-010-9245-4

Abstract

Mycobacterium tuberculosis is the most common communicable infectious disease worldwide and the top killer of human immunodeficiency virus (HIV)-infected people. Because of common dual HIV and M. tuberculosis infections, the emergence of multidrug-resistant M. tuberculosis strains, the lack of effective vaccination, the morbidity, and the mortality of M. tuberculosis infection are increasing sharply. Therefore, there is an urgent need for vaccine and drug development against M. tuberculosis infection. These require appropriate animal models that closely resemble human disease. To this end, we infected Chinese rhesus macaques with the M. tuberculosis H37Rv strain. Bronchoscopy was used to inoculate nine monkeys with different doses of M. tuberculosis H37Rv strain. Regardless of the M. tuberculosis dose, all monkeys were infected successfully. This was shown by clinical, laboratory, and histopathology assessments. Among nine infected monkeys, six developed acute rapid progressive tuberculosis and the remaining animals mirrored early-stage chronic disease. These data, taken together, demonstrate that Chinese rhesus macaques are highly susceptible to M. tuberculosis infection and develop similar manifestations of disease that are seen in humans. This model affords new opportunities for studies of M. tuberculosis disease pathology and therapeutics.

Keywords

Mycobacterium tuberculosisNon-human primatesChinese rhesus macaques

Abbreviations

ABSL

Animal biosafety level

CRP

C-reactive protein

CFU

Colony forming units

ESR

Erythrocyte sedimentation rate

OT

Old tuberculin

PI

Post-inoculation

SIV

Simian immunodeficiency virus

TST

Tuberculin skin test

TB

Tuberculosis

WHO

World Health Organization

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Jing Zhang
    • 1
  • Yan-Qing Ye
    • 2
  • Yong Wang
    • 1
  • Ping-Zheng Mo
    • 2
  • Qiao-Yang Xian
    • 1
  • Yan Rao
    • 1
  • Rong Bao
    • 1
  • Ming Dai
    • 1
  • Jun-Yan Liu
    • 1
  • Ming Guo
    • 1
  • Xin Wang
    • 1
  • Zhi-Xiang Huang
    • 1
  • Li-Hua Sun
    • 1
  • Zhi-Jiao Tang
    • 1
  • Wen-Zhe Ho
    • 1
    • 3
  1. 1.ABSL-3 LaboratoryWuhan UniversityHubeiPeople’s Republic of China
  2. 2.Department of RespirationZhongnan Hospital, Wuhan UniversityHubeiPeople’s Republic of China
  3. 3.Department of Pathology and Laboratory MedicineTemple University School of MedicinePhiladelphiaUSA