Plasmonics

, Volume 2, Issue 4, pp 201–215

Plasmonic DNA: Towards Genetic Diagnosis Chips

  • Jérôme Hottin
  • Julien Moreau
  • Gisèle Roger
  • Jolanda Spadavecchia
  • Marie-Claude Millot
  • Michel Goossens
  • Michael Canva
Article

DOI: 10.1007/s11468-007-9039-6

Cite this article as:
Hottin, J., Moreau, J., Roger, G. et al. Plasmonics (2007) 2: 201. doi:10.1007/s11468-007-9039-6

Abstract

The present paper summarizes some of our activities in the field of plasmonic DNA and genetic diagnosis, presenting our system and its capabilities before showing data related to the design and use of functionalized biochips of increasing complexity along with various experimental hybridization conditions, including solutions containing one type of purified synthetic short oligonucleotides or PCR-amplified DNA samples from patients. The diagnosis capability of our system was evaluated by detecting several point mutations that alter the function of the CFTR gene and cause cystic fibrosis, a frequent monogenic disorder selected as a clinical model system.

Keywords

Surface plasmon resonance imaging (SPRI) DNA sensor DNA–DNA interactions Surface functionalization Mutation Genetic diagnosis 

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Jérôme Hottin
    • 1
  • Julien Moreau
    • 1
  • Gisèle Roger
    • 1
  • Jolanda Spadavecchia
    • 1
  • Marie-Claude Millot
    • 2
  • Michel Goossens
    • 3
  • Michael Canva
    • 1
  1. 1.Laboratoire Charles Fabry de l’Institut d’Optique (LCFIO), Institut d’Optique, Graduate schoolUniv Paris SudPalaiseau CedexFrance
  2. 2.Équipe Systèmes Polymères Complexes (SCP), Institut de Chimie et des Matériaux Paris EstUniversité Paris 12ThiaisFrance
  3. 3.Plate-forme de Génomique IFR10Institut National de la Santé et de la Recherche Médicale (INSERM)CréteilFrance

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