, Volume 57, Issue 26, pp 3525-3531,
Open Access This content is freely available online to anyone, anywhere at any time.
Date: 06 May 2012

The interaction of GSSG modified magnetic nanoparticles with SPC-A1 cells in vitro

Abstract

Magnetic nanoparticles (MNPs) are promising materials for various biomedical applications, including magnetic resonance imaging, stem cell tracking, gene/drug delivery, and cancer treatment. To increase the effectiveness of MNPs, high capture efficiency and controlled uptake of the particles by cells is required. In this paper we report the cytotoxicity and cellular uptake into SPC-A1 cells of oxidized glutathione (GSSG)-modified MNPs (GSSG@Fe3O4). Experimental findings indicated that GSSG@Fe3O4 were biocompatible, and could be efficiently taken up by SPC-A1 cells (up to 160 pg iron per cell). The internalized GSSG@Fe3O4 was retained in the cell cytoplasm for 6 generations. The uptake of GSSG@Fe3O4 into SPC-A1 cells was energy-, concentration- and time-dependent. Pinocytosis may be involved in the internalization process of GSSG@Fe3O4 into SPC-A1 cells, but this mechanism remains to be elucidated. The controlled and efficient localization of GSSG@Fe3O4 into the cytosol and long intracellular retention provides theoretical and experimental insight into the biomedical applications for these molecules.

This article is published with open access at Springerlink.com