Chinese Science Bulletin

, 54:1204

Enhanced validamycin production and gene expression at elevated temperature in Streptomyces hygroscopicus subsp. jingangensis 5008


DOI: 10.1007/s11434-009-0128-0

Cite this article as:
Li, L., Bai, L., Zhou, X. et al. Chin. Sci. Bull. (2009) 54: 1204. doi:10.1007/s11434-009-0128-0


Cultivation shift from 30° to 37° significantly enhanced validamycin (VAL) production. Analyzed by reverse-transcription PCR, the transcription of three val genes, valA, valK and valG, representing the three operons of the cluster was simultaneously increased at elevated temperature. Furthermore, the transcription of valP and valQ, a pair of two-component regulators in validamycin biosynthetic gene cluster, was also increased at 37°. Inactivation of valP and valQ reduced validamycin production at 37° to the yield level of wild type strain at 30°, and the val genes showed reduced expression in the mutant LL-8 at 37°. These results revealed that the two-component regulator valP and valQ contribute to the elevated validamycin production.


Streptomyces validamycin regulation enhanced production 

Copyright information

© Science in China Press and Springer-Verlag GmbH 2009

Authors and Affiliations

  1. 1.School of Life Science & Biotechnology and Laboratory of Microbial MetabolismShanghai Jiao Tong UniversityShanghaiChina

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