Chinese Science Bulletin

, Volume 53, Issue 3, pp 370–376

Effects of vascular endothelial growth factor on angiogenesis of the endothelial cells isolated from cavernous malformations

  • Tan YuZhen 
  • Zhao Yao 
  • Wang HaiJie 
  • Zhou LiangFu 
  • Mao Ying 
  • Liu Rui 
  • Shu Jia 
  • Wang YongFei 
Articles Biomedical Engineering

DOI: 10.1007/s11434-008-0121-z

Cite this article as:
Tan, Y., Zhao, Y., Wang, H. et al. Chin. Sci. Bull. (2008) 53: 370. doi:10.1007/s11434-008-0121-z

Abstract

Human cerebral cavernous malformation (CM) is a common vascular malformation of the central nervous system. We have investigated the biological characteristics of CM endothelial cells and the cellular and molecular mechanisms of CM angiogenesis to offer new insights into exploring effective measures for treatment of this disease. The endothelial cells were isolated from CM tissue masses dissected during operation and expanded in vitro. Expression of VEGFR-1 and VEGFR-2 was examined with immunocytochemical staining. Proliferation, migration and tube formation of CM endothelial cells were determined using MTT, wounding and transmigration assays, and three-dimensional collagen type I gel respectively. The endothelial cells were successfully isolated from the tissue specimens of 25 CMs dissected without dipolar electrocoagulation. The cells show the general characteristics of the vascular endothelial cells. Expression of VEGFR-1 and VEGFR-2 on the cells is higher than that on the normal cerebral microvascular endothelial cells. After treatment with VEGF, numbers of the proliferated and migrated cells, the maximal distance of cell migration and the length and area of capillary-like structures formed in the three-dimensional collagen gel increase significantly. These results demonstrate that expression of VEGFR-1 and VEGFR-2 on CM endothelial cells is up-regulated. By binding to receptors, VEGF may activate the downstream signaling pathways and promote proliferation, migration and tube formation of CM endothelial cells. VEGF/VEGFR signaling pathways play important regulating roles in CM angiogenesis.

Keywords

cavernous malformationendothelial cellsVEGFVEGFR-1VEGFR-2angiogenesis

Copyright information

© Science in China Press 2008

Authors and Affiliations

  • Tan YuZhen 
    • 1
  • Zhao Yao 
    • 2
  • Wang HaiJie 
    • 1
  • Zhou LiangFu 
    • 2
  • Mao Ying 
    • 2
  • Liu Rui 
    • 1
  • Shu Jia 
    • 1
  • Wang YongFei 
    • 2
  1. 1.Department of Anatomy and Histology and EmbryologyShanghai Medical School of Fudan UniversityShanghaiChina
  2. 2.Department of Neurosurgery, Huashan HospitalFudan UniversityShanghaiChina