Chinese Science Bulletin

, Volume 52, Issue 16, pp 2203–2209

Expression patterns of the rice class I metallothionein gene family in response to lead stress in rice seedlings and functional complementation of its members in lead-sensitive yeast cells

Authors

  • Xu YuFeng 
    • Laboratory of Molecular Biology and Protein Science Key Laboratory of the Ministry of Education, Department of Biological Sciences and BiotechnologyTsinghua University
  • Zhou GongKe 
    • Laboratory of Molecular Biology and Protein Science Key Laboratory of the Ministry of Education, Department of Biological Sciences and BiotechnologyTsinghua University
  • Zhou Lu 
    • Laboratory of Molecular Biology and Protein Science Key Laboratory of the Ministry of Education, Department of Biological Sciences and BiotechnologyTsinghua University
  • Li YiQin 
    • Laboratory of Molecular Biology and Protein Science Key Laboratory of the Ministry of Education, Department of Biological Sciences and BiotechnologyTsinghua University
    • Laboratory of Molecular Biology and Protein Science Key Laboratory of the Ministry of Education, Department of Biological Sciences and BiotechnologyTsinghua University
Articles Plant Physiology

DOI: 10.1007/s11434-007-0335-5

Cite this article as:
Xu, Y., Zhou, G., Zhou, L. et al. CHINESE SCI BULL (2007) 52: 2203. doi:10.1007/s11434-007-0335-5

Abstract

Metallothioneins (MTs) are a group of low molecular mass and cysteine-rich proteins that can chelate heavy-metal ions. In this paper, Northern blot analysis was used to investigate the influence of lead stress on the expression patterns of 10 rice class I MT genes (OsMT-Is) in rice seedlings. With the exception of OsMT-I-3b, the data demonstrate dynamic changes of 9 OsMT-I transcripts in response to Pb2+ treatment in rice seedling roots. Of these genes, transcription of OsMT-I-1a, OsMT-I-1b, OsMT-I-2c, OsMT-I-4a, OSMT-I-4b and OsMT-I-4c increased significantly, while transcription of OsMT-I-2a and OsMT-I-3a increased marginally. In contrast, the expression of OsMT-I-2b was inhibited. Pb2+ induced the expression of 6 OsMT-I genes in seedling shoots, but had no obvious effects on the expression of OsMT-I-1a, OsMT-I-1b, OsMT-I-4a and OsMT-I-4b. All the 10 OsMT-Is had enhanced lead tolerance when heterologously expressed in lead-sensitive yeast mutant cells. These results provide an expression profile of the rice MT gene family in response to Pb2+ stress in rice seedlings and demonstrate increased lead tolerance in sensitive yeast mutant cells expressing OsMT-Is. This study lays a foundation for further analysis of the role of the rice MT gene family in respond to Pb2+ stress.

Keywords

rice metallothionein gene family Pb2+ induced expression yeast tolerance

Copyright information

© Science in China Press 2007