Chinese Science Bulletin

, Volume 52, Issue 16, pp 2216–2225

Induction of corneal epithelial progenitors from bone-marrow mesenchymal stem cells of rhesus monkeys in vitro

  • Yuan Jing 
  • Yu JianXiong 
  • Huang Bing 
  • Liu BingQian 
  • Liu JingBo 
  • Jiang RuZhang 
  • Ge Jian 
Articles Cell Biology

DOI: 10.1007/s11434-007-0304-z

Cite this article as:
Yuan, J., Yu, J., Huang, B. et al. CHINESE SCI BULL (2007) 52: 2216. doi:10.1007/s11434-007-0304-z

Abstract

Bioengineered corneas are substitutes for human donor tissue that are designed to treat severe disease affecting ocular surfaces. However, a shortage of candidate seed cells for bioengineering corneas is still a problem. Bone-marrow mesenchymal stem cells (MSCs) are capable of multilineage differentiation. Therefore, we determined whether MSCs differentiate into corneal epithelial cells (ECs). We applied three exoteric-microenvironmental systems to induce MSCs to become ECs. Induced MSC were identified by means of morphologic examination, immunocytochemical analysis, and flow cytometry. MSCs grown in one microenvironment had characteristics similar to those of corneal epithelial progenitors. Induced MSCs expressed markers for EC, including integrin β1, Cx43, Pax6, and P63. MSCs were successfully induced to become corneal epithelial progenitors. Therefore, the use of MSCs may hold substantial promise for reconstructing the ocular surface after corneal injury.

Keywords

rhesus monkey bone-marrow mesenchymal stem cell corneal epithelial cells denuded amniotic membrane Transwell system growth factor Lipopolysaccharid 

Copyright information

© Science in China Press 2007

Authors and Affiliations

  • Yuan Jing 
    • 1
    • 2
  • Yu JianXiong 
    • 3
  • Huang Bing 
    • 1
  • Liu BingQian 
    • 1
  • Liu JingBo 
    • 1
  • Jiang RuZhang 
    • 1
    • 2
  • Ge Jian 
    • 1
    • 2
  1. 1.State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhouChina
  2. 2.Center for Stem Cell Biology and Tissue EngineeringSun Yat-sen UniversityGuangzhouChina
  3. 3.Department of General SurgeryThe Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouChina

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