Theoretical investigation on Raman induced kerr effect spectroscopy in nonlinear confocal microscopy

Article

DOI: 10.1007/s11433-008-0086-6

Cite this article as:
Guo, L., Tang, Z. & Xing, D. Sci. China Ser. G-Phys. Mech. Astron. (2008) 51: 788. doi:10.1007/s11433-008-0086-6

Abstract

The imaging theory of Raman induced Kerr effect spectroscopy (RIKES) in nonlinear confocal microscopy is presented in this paper. Three-dimensional point spread function (3D-PSF) of RIKES nonlinear confocal microscopy in isotropic media is derived with Fourier imaging theory and RIKES theory. The impact of nonlinear property of RIKES on the spatial resolution and imaging properties of confocal microscopy have been analyzed in detail. It is proved that RIKES nonlinear confocal microscopy can simultaneously provide more information than two-photon confocal microscopy concerning molecular vibration mode, vibration orientation and optically induced molecular reorientation, etc. It is shown that RIKES nonlinear confocal microscopy significantly enhances the spatial resolution and imaging quality of confocal microscopy and achieves much higher resolution than that of two-photon confocal microscopy.

Keywords

nonlinear confocal microscopy Raman induced Kerr effect spectroscopy three-dimensional point spread function 

Copyright information

© Science in China Press and Springer-Verlag GmbH 2008

Authors and Affiliations

  1. 1.School of Physics and Telecommunication EngineeringSouth China Normal UniversityGuangzhouChina
  2. 2.Key Laboratory of Laser Life Science, Ministry of Education of ChinaSouth China Normal UniversityGuangzhouChina

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