In order to discuss the values and daily variation characteristics of heat storage fluxes in a tropical seasonal rain forest in Xishuangbanna, the sensible and latent heat storage flux within air column, canopy heat storage flux, energy storage by photosynthesis and ground heat storage above the soil heat flux plate, as well as the ratios of these heat storage fluxes to the net radiation in the cool-dry, hot-dry and rainy season were compared and analyzed based on the observation data of carbon fluxes, meteorological factors and biomass within this tropical seasonal rain forest from January 2003 to December 2004. The findings showed that heat storage terms ranged significantly in the daytime and weakly in the nighttime, and the absolute values of sensible and latent heat storage fluxes were obviously greater than other heat storage terms in all seasons. In addition, the absolute values of total heat storage fluxes reached the peak in the hot-dry season, then were higher in the rainy season, and reached the minimum in the cool-dry season. The ratios of heat storage fluxes to net radiation generally decreased with time in the daytime, moreover, the sensible and latent heat storage dominated a considerable fraction of net radiation, while other heat storage contents occupied a smaller fraction of the net radiation and the peak value was not above 3.5%. In the daytime, the ratios of the total heat storage to net radiation were greater and differences in these ratios were distinct among seasons before 12:00, and then they became lower and differences were small among sea-sons after 12:00. The energy closure was improved when the storage terms were considered in the energy balance, which indicated that heat storage terms should not been neglected. The energy closure of tropical seasonal rain forest was not very well due to effects of many factors. The results would help us to further understand energy transfer and mass exchange between tropical forest and atmosphere. Moreover, they would supply a research basis for studying energy closure at other places.