Science China Life Sciences

, Volume 57, Issue 2, pp 221–231

Investigating design principles of micropatterned encapsulation systems containing high-density microtissue arrays

  • LiYang Jiang
  • JiaYing Liu
  • Kai Wang
  • Xi Gu
  • Ying Luo
Open AccessResearch Paper Thematic Issue: Stem Cells and Regenerative Medicine in China

DOI: 10.1007/s11427-014-4609-2

Cite this article as:
Jiang, L., Liu, J., Wang, K. et al. Sci. China Life Sci. (2014) 57: 221. doi:10.1007/s11427-014-4609-2
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Abstract

Immunoisolation is an important strategy to protect transplanted cells from rejection by the host immune system. Recently, microfabrication techniques have been used to create hydrogel membranes to encapsulate microtissue in an arrayed organization. The method illustrates a new macroencapsulation paradigm that may allow transplantation of a large number of cells with microscale spatial control, while maintaining an encapsulation device that is easily maneuverable and remaining integrated following transplantation. This study aims to investigate the design principles that relate to the translational application of micropatterned encapsulation membranes, namely, the control over the transplantation density/quantity of arrayed microtissues and the fidelity of pre-formed microtissues to micropatterns. Agarose hydrogel membranes with microwell patterns were used as a model encapsulation system to exemplify these principles. Our results show that high-density micropatterns can be generated in hydrogel membranes, which can potentially maximize the percentage volume of cellular content and thereby the transplantation efficiency of the encapsulation device. Direct seeding of microtissues demonstrates that microwell structures can efficiently position and organize pre-formed microtissues, suggesting the capability of micropatterned devices for manipulation of cellular transplants at multicellular or tissue levels. Detailed theoretical analysis was performed to provide insights into the relationship between micropatterns and the transplantation capacity of membrane-based encapsulation. Our study lays the ground for developing new macroencapsulation systems with microscale cellular/tissue patterns for regenerative transplantation.

Keywords

hydrogelmicropatternarraymulti-cellular spheroidsmacroencapsulation
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Supplementary material

11427_2014_4609_MOESM1_ESM.pdf (328 kb)
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Copyright information

© The Author(s) 2014

Authors and Affiliations

  • LiYang Jiang
    • 1
  • JiaYing Liu
    • 1
  • Kai Wang
    • 1
  • Xi Gu
    • 1
  • Ying Luo
    • 1
    • 2
  1. 1.Department of Biomedical Engineering, College of EngineeringPeking UniversityBeijingChina
  2. 2.National Engineering Laboratory for Regenerative and Implantable Medical DevicesGuangzhouChina