Science China Life Sciences

, Volume 57, Issue 2, pp 201–208

Effects of cannabinoid receptor type 2 on endogenous myocardial regeneration by activating cardiac progenitor cells in mouse infarcted heart

  • YaBin Wang
  • Sai Ma
  • Qiang Wang
  • WenXing Hu
  • DongJuan Wang
  • XiuJuan Li
  • Tao Su
  • Xing Qin
  • XiaoTian Zhang
  • Ke Ma
  • JiangWei Chen
  • LiZe Xiong
  • Feng Cao
Open AccessResearch Paper Thematic Issue: Stem cells and regenerative medicine in China

DOI: 10.1007/s11427-013-4604-z

Cite this article as:
Wang, Y., Ma, S., Wang, Q. et al. Sci. China Life Sci. (2014) 57: 201. doi:10.1007/s11427-013-4604-z

Abstract

Cannabinoid receptor type 2 (CB2) activation is recently reported to promote proliferation of some types of resident stem cells (e.g., hematopoietic stem/progenitor cell or neural progenitor cell). Resident cardiac progenitor cell (CPC) activation and proliferation are crucial for endogenous cardiac regeneration and cardiac repair after myocardial infarction (MI). This study aims to explore the role and possible mechanisms of CB2 receptor activation in enhancing myocardial repair. Our results revealed that CB2 receptor agonist AM1241 can significantly increase CPCs by c-kit and Runx1 staining in ischemic myocardium as well as improve cardiomyocyte proliferation. AM1241 also decreased serum levels of MDA, TNF-α and IL-6 after MI. In addition, AM1241 can ameliorate left ventricular ejection fraction and fractional shortening, and reduce fibrosis. Moreover, AM1241 treatment markedly increased p-Akt and HO-1 expression, and promoted Nrf-2 nuclear translocation. However, PI3K inhibitor wortmannin eliminated these cardioprotective roles of AM1241. In conclusion, AM1241 could induce myocardial regeneration and improve cardiac function, which might be associated with PI3K/Akt/Nrf2 signaling pathway activation. Our findings may provide a promising strategy for cardiac endogenous regeneration after MI.

Keywords

CB2 receptor cardiac progenitor cells endogenous cardiac regeneration 
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© The Author(s) 2014

Authors and Affiliations

  • YaBin Wang
    • 1
  • Sai Ma
    • 1
  • Qiang Wang
    • 2
  • WenXing Hu
    • 1
  • DongJuan Wang
    • 1
  • XiuJuan Li
    • 1
  • Tao Su
    • 1
  • Xing Qin
    • 1
  • XiaoTian Zhang
    • 1
  • Ke Ma
    • 1
  • JiangWei Chen
    • 1
  • LiZe Xiong
    • 2
  • Feng Cao
    • 1
    • 3
  1. 1.Department of Cardiology, Xijing HospitalFourth Military Medical UniversityXi’anChina
  2. 2.Department of Anesthesiology, Xijing HospitalFourth Military Medical UniversityXi’anChina
  3. 3.Department of CardiologyPLA General HospitalBeijingChina