Science China Life Sciences

, Volume 57, Issue 2, pp 162–170

Neural progenitor cells from human induced pluripotent stem cells generated less autogenous immune response

  • Ke Huang
  • PengFei Liu
  • Xiang Li
  • ShuBin Chen
  • LiHui Wang
  • Li Qin
  • ZhengHui Su
  • WenHao Huang
  • JuLi Liu
  • Bei Jia
  • Jie Liu
  • JingLei Cai
  • DuanQing Pei
  • GuangJin Pan
Open AccessResearch Paper Thematic Issue: Stem cells and regenerative medicine in China

DOI: 10.1007/s11427-013-4598-6

Cite this article as:
Huang, K., Liu, P., Li, X. et al. Sci. China Life Sci. (2014) 57: 162. doi:10.1007/s11427-013-4598-6

Abstract

The breakthrough development of induced pluripotent stem cells (iPSCs) raises the prospect of patient-specific treatment for many diseases through the replacement of affected cells. However, whether iPSC-derived functional cell lineages generate a deleterious immune response upon auto-transplantation remains unclear. In this study, we differentiated five human iPSC lines from skin fibroblasts and urine cells into neural progenitor cells (NPCs) and analyzed their immunogenicity. Through co-culture with autogenous peripheral blood mononuclear cells (PBMCs), we showed that both somatic cells and iPSC-derived NPCs do not stimulate significant autogenous PBMC proliferation. However, a significant immune reaction was detected when these cells were co-cultured with allogenous PBMCs. Furthermore, no significant expression of perforin or granzyme B was detected following stimulation of autogenous immune effector cells (CD3+CD8 T cells, CD3+CD8+ T cells or CD3CD56+ NK cells) by NPCs in both PBMC and T cell co-culture systems. These results suggest that human iPSC-derived NPCs may not initiate an immune response in autogenous transplants, and thus set a base for further preclinical evaluation of human iPSCs.

Keywords

induced pluripotent stem cellsimmunogenicityiPSC-derived neural progenitor cells
Download to read the full article text

Copyright information

© The Author(s) 2014

Authors and Affiliations

  • Ke Huang
    • 1
  • PengFei Liu
    • 1
    • 2
  • Xiang Li
    • 1
  • ShuBin Chen
    • 1
  • LiHui Wang
    • 1
  • Li Qin
    • 3
  • ZhengHui Su
    • 1
  • WenHao Huang
    • 1
  • JuLi Liu
    • 1
  • Bei Jia
    • 4
  • Jie Liu
    • 4
  • JingLei Cai
    • 1
  • DuanQing Pei
    • 1
  • GuangJin Pan
    • 1
  1. 1.Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhouChina
  2. 2.Department of Regeneration Medicine, School of Pharmaceutical ScienceJilin UniversityChangchunChina
  3. 3.Laboratory of Pathogen Biology, State Key Laboratory of Respiratory Disease, Center for Infection and Immunity, Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhouChina
  4. 4.Department of Obstetrics and Gynecology, Nanfang HospitalSouthern Medical UniversityGuangzhouChina