Science China Life Sciences

, Volume 56, Issue 6, pp 531–540

Immunogenicity analysis following human immunodeficiency virus recombinant DNA and recombinant vaccinia virus Tian Tan prime-boost immunization

  • CunXia Liu
  • ShouWen Du
  • Chang Li
  • YuHang Wang
  • MaoPeng Wang
  • Yi Li
  • RongLan Yin
  • Xiao Li
  • DaYong Ren
  • YanQing Qin
  • JingQiang Ren
  • NingYi Jin
Open Access
Research Paper

DOI: 10.1007/s11427-013-4484-2

Cite this article as:
Liu, C., Du, S., Li, C. et al. Sci. China Life Sci. (2013) 56: 531. doi:10.1007/s11427-013-4484-2

Abstract

This study assessed and compared the immunogenicity of various immunization strategies in mice using combinations of recombinant DNA (pCCMp24) and recombinant attenuated vaccinia virus Tian Tan (rddVTT-CCMp24). Intramuscular immunization was performed on days 0 (prime) and 21 (boost). The immunogenicity of the vaccine schedules was determined by measuring human immunodeficiency virus (HIV)-specific binding antibody levels and cytokine (interleukin-2 and interleukin-4) concentrations in peripheral blood, analyzing lymphocyte proliferation capacity against HIV epitopes and CD4+/CD8+ cell ratio, and monitoring interferon-gamma levels at different times post-immunization. The results showed that pCCMp24, rddVTT-CCMp24 and their prime-boost immunization induced humoral and cellular immune responses. The pCCMp24/rddVTT-CCMp24 immunization strategy increased CD8+ T cells and induced more IFN-γ-secreting cells compared with single-shot rDNA. The prime-boost immunization strategy also induced the generation of cellular immunological memory to HIV epitope peptides. These results demonstrated that prime-boost immunization with rDNA and rddVTT-CCMp24 had a tendency to induce greater cellular immune response than single-shot vaccinations, especially IFN-γ response, providing a basis for further studies.

Keywords

vaccinia virus Tian Tan human immunodeficiency virus recombinant DNA prime-boost immunogenicity 
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Copyright information

© The Author(s) 2013

Authors and Affiliations

  • CunXia Liu
    • 1
    • 2
  • ShouWen Du
    • 1
    • 2
  • Chang Li
    • 2
  • YuHang Wang
    • 2
  • MaoPeng Wang
    • 1
    • 2
  • Yi Li
    • 2
  • RongLan Yin
    • 3
  • Xiao Li
    • 2
  • DaYong Ren
    • 1
    • 2
  • YanQing Qin
    • 2
  • JingQiang Ren
    • 1
    • 2
  • NingYi Jin
    • 2
  1. 1.College of Animal Science and Veterinary MedicineJilin UniversityChangchunChina
  2. 2.Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military VeterinaryAMMSChangchunChina
  3. 3.Academy of Animal Science and Veterinary Medicine in Jilin ProvinceChangchunChina

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