Research Paper

Science China Life Sciences

, Volume 55, Issue 11, pp 968-973

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Atomic force microscopy imaging and mechanical properties measurement of red blood cells and aggressive cancer cells

  • Mi LiAffiliated withState Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of SciencesUniversity of Chinese Academy of SciencesState Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
  • , LianQing LiuAffiliated withState Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences Email author 
  • , Ning XiAffiliated withState Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of SciencesDepartment of Mechanical and Biomedical Engineering, City University of Hong Kong Email author 
  • , YueChao WangAffiliated withState Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences
  • , ZaiLi DongAffiliated withState Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences
  • , XiuBin XiaoAffiliated withDepartment of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences
  • , WeiJing ZhangAffiliated withDepartment of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences Email author 

Abstract

Mechanical properties play an important role in regulating cellular activities and are critical for unlocking the mysteries of life. Atomic force microscopy (AFM) enables researchers to measure mechanical properties of single living cells under physiological conditions. Here, AFM was used to investigate the topography and mechanical properties of red blood cells (RBCs) and three types of aggressive cancer cells (Burkitt’s lymphoma Raji, cutaneous lymphoma Hut, and chronic myeloid leukemia K562). The surface topography of the RBCs and the three cancer cells was mapped with a conventional AFM probe, while mechanical properties were investigated with a micro-sphere glued onto a tip-less cantilever. The diameters of RBCs are significantly smaller than those of the cancer cells, and mechanical measurements indicated that Young’s modulus of RBCs is smaller than those of the cancer cells. Aggressive cancer cells have a lower Young’s modulus than that of indolent cancer cells, which may improve our understanding of metastasis.

Keywords

atomic force microscopy red blood cell cancer cell mechanical properties Young’s modulus